#aneuploids

[cytgen]

Abstract In this experiment the development of the various chromosomal interchanges in Sowa (Anethum graveolens L.) was accomplished with the help of Gamma irradiations. The pollen mother cells of Anethum graveolens were observed to be perfectly normal in untreated plants and displayed a regular formation of eleven bivalents at diakinesis, followed by normal separation (11:11) at anaphase I. Cytological manifestation of chromosome configurations at diakinesis and metaphase-I exhibited translocation heterozygotes by the formation of either ring or chain of chromosomes in PMCs of Sowa, particularly at higher doses of Gamma irradiation (200 Gy). The translocation lines showed discernible prevalence of rings (56.58%) over chains (43.42%). In chromosomal configuration, PMCs of Irradiated plants, shows the presence of minimum one or more quadrivalent and bivalents, besides this, some PMCs showed other configurations such as trivalents, pentavalents, hexavalent, and octavalent along with variable number of univalents. At diakinesis, stage of PMCs, 2III + 1IX + 2IV + 1II + 1I configuration was observed in maximum (12.88%) followed by 6II + 1IV + 1V + 1I (12.37%) and 1VII + 1V + 2IV + 2I (11.85%). The configuration 1X + 2IV + 2II showed the lowest (5.15%) frequency. However, a variety of anomalies such as unequal separation (34.93%), laggards (21.91%) and bridges (12.32%) were also recorded. Pollen fertility was reduced (41.21 ± 0.19%) in translocation lines as comparison to control plant (98.59 ± 0.25%). Translocation heterozygotes might be used as an initial foundation for developing aneuploids with novel gene combinations.

[mindblowingscience]

How we classify cancer and spot it in its earliest stages could need an urgent rethink: researchers have found that even some healthy women carry cells with the key hallmarks of breast cancer. These cells are known as aneuploid cells, and have an abnormal number of chromosomes. They're common in invasive breast cancer, and it's thought the chromosome imbalance enables cancer to spread and evade the body's immune defenses.

Continue Reading.

[orphancookie69]

My Infertility Journey: Part 6

There are so many things I thought I would not do again and yet here I am. Hope is a dangerous thing. Eggs are here, now the question of...Should I carry or not?

To Catch Up:

Part 5

Part 4 

Part 3 

Part 2 

Part 1 

October 2023:

So. Egg retrieval came, eggs went to testing, body came back. The body when it comes back comes back sooner and with a vengeance. The body feels like it did not complete the first one because the eggs were taken out and it starts again. That cycle tends to be one of the heavier ones. Doctor put me on Birth Control, Enskyce, to calm the factory down while we wait for the results of the embryo grading. I also transferred my embryo from Egg Retrieval #1 to join its new sibling embryo at the new office. Embryo transfer was $250, and a little nerve racking.

My partner goes to a new chiropractor, his wife specializes in fertility. My partner forced me to speak to her, Dr Alicia McDonough of Good Health & Wellness Chiropractic. Partners out there, don't force your partner to do something-let them do it when it makes sense to them and trust them. Anyways, here are the results of the consultation:

What is red light therapy? From what she said, it was using lights to increase blood flow to help heal the body naturally. Need to look more into this. 

Do I consume 120-130 grams of protein per day? I ran the numbers, my normal routine put me at about 100. By doubling up the amount of protein powder in my AM/Lunch smoothie, I hit 120, and any other snacks would be protein bonuses. 

Adding resistance training? I do 30 minutes a day, I need to look at what that is resistance training and possibly up 30 to 45 minutes by adding 15 minutes of resistance training. 

Trading CoQ10 for Ubiquinol? I am on CoQ10 as a prenatal but also help with migraine management. I was told Ubiquinol is the more effective version of that, apparently vitamins are the tip of the iceberg and supplements are better than vitamins. I was told Ubiquinol was sold at Coscto, but I did not see it. This makes for better egg quality, which assumes a third round of making eggs in the future. 

DHEA testing, and MTHFR testing? Yes. How did these tests get missed? 

Future possibility: track my own cycle at home with ovation sticks (determine my cycle peak), track my temperature in the morning (figure out if I run warm or cold), get the endometriosis confirmed and cleaned up surgically (how and who does that?), Recover, Use red light therapy, and try to have kids on my own on a year off if IVF fails? To confirm endo: Laparoscopy and laparotomy. Recovery is usually 6 weeks (almost 2 months). 

Future Possibility #2: If Egg Retrieval #2 does not work, maybe I should focus on egg quality vs quantity. Question then becomes, do I work with Dr. Mcdonough on that? Does she tell me what to tell my IVF doctor? Is that IVF? 

Present Question: If my eggs are indicative of what I was doing 4 months ago, was I in an ok place 4 months ago for ER #2? I started low fodmap and exercise in July, ER #2 was in September, so May? Maybe. 

Past Question: Based on the 4 month assumption being right, what about ER #1? In 2020? Are low quality eggs why no embryo from ER#1 has made it? I transferred the last embryo from ER #1, is it worth implanting then?

The consultation was $50. She mentioned mindset being important, and not thinking you are the issue. This is where I am going to disagree with her, while I would not have chosen to be this burden on my partner financially and every other way-there is a small part of this I can own. Once you own it, you can take control of it. I could of doubted my parent and checked it out myself. She mentioned weight not being an issue, while that was nice to hear-its not entirely true. We agreed a strong body can be a healthy body and the overall weight number isn't the end all be all. It was interesting, but not much help on the current path. More a future path, but I am too close to the current path to see beyond the woods.

Back to the current path, the enskyce started to give me migraines and nausea. I switched, with approval, and waited for the PGT results. Once those are in, we do an uterine evaluation and endoscratch prior to my transfer cycle. We received the results: the one embryo that made it was abnormal/aneuploid. We had a follow up appointment with our doctor to discuss next steps.

We spoke with Dr J Lin. He was shocked at our results. I was told to stop taking Birth Control, and call on the first day of my next cycle. Based on Egg Retrieval #2 results, and looking at an ultrasound today-it was advised to check back in after a month of no hormones to start Egg Retrieval #3. Egg Retrieval #3 would be longer (despite the fact that ER#2 was already longer than normal) and more days of Menopur. Financial department would call me with an estimate for ER#3 and I was advised to take the prenatal vitamins and supplements-as he confirmed your eggs are a reflection of the last 3 months. I stopped Birth Control. I also started to take my temperature daily and using Premom app/thermometer to determine some of my own data. I also started to do at home: red light therapy, cervical spine traction device, and a tens machine.

November 2023:

Next cycle was estimated for 4 weeks from the appointment-around the 4th week of November. Between the holidays and everything else-we decided to take the rest of the year off. It was nice to be able to enjoy the holidays.

December 2023:

I started the learning curve of gathering and understanding the data from my cycles using daily Basal Body Temperature checks and LH checks. In October, I also initiated the transfer of Eggs from ER#1 to join the eggs from ER#2 and found out that never got completed. So we decided in January to revisit that when everyone was back to work.

January 2024:

New year, same fight. I gathered 3 months of data using Premom, it is pretty interesting. We also picked up where we left off in the Egg Transfer. I forgot how many abnormal embryos I had, and wondered why they were even being stored if no one really had a good argument for implanting them? My partner wanted to keep them, based on a suggestion from a family friend, but honestly it did not make sense to me. But, as they are my eggs-we had to choose to dissolve them and sign the paperwork to do so.

In my head there were a couple of things at play. First of all, this would be year 5 or year 6 of trying to have a kid, and I was really worried about doing this for 10 years and having nothing to show for it and having that change me (for the worse). That being said, I have yet to give up so the options were: try an implant with the new office, implant an embryo into a stranger, or try for more eggs. While I know, if I choose to continue this journey I can't avoid another ER, getting 0 from ER#2 was (and still does) suck. Once we finally had the eggs of play in place, we met with the doctor to discuss next steps.

February 2024:

So it was arranged for Cryoport transported my embryos from Ovation Fertility in Newport Beach to Reproductive Fertility Care in Irvine, CA. Transport went well, thank goodness. Next order of business was to dissolve the abnormals at Ovation Fertility. I was quite torn about dissolving them. But its hard to make a case to keep them. I closed out the account, and I am glad I did. The way that company acted towards me, with my objects, was not ok.

March 2024:

I was put on Birth Control to calm my system down and on the day we would have implanted on that cycle, we decided to do a Mock Embryo Transfer, SIS, and Ultrasound. That went well. Upon the next cycle start, we set an implant date for the last normal embryo in our storage.

April 2024:

Next cycle came and we did a prenatal blood draw and ultrasound. The ultrasound looked good. Started taking a mix of pills, patches, injections, and suppositories. At first there were no injections, which was nice time to prepare the body for them. Closer to implant the injections started in the back for progesterone and in the front for blood thinners. We also did 1 round of Intralipids before the implant. I was going to implant both my mosaic and normal but ended up being talked out of it. On implant day we arrived 30 minutes early, I started filling up my bladder 1 hour before the actual appointment time. Implant went perfectly. Came home and rested and eased into my new normal. Went in for the blood test almost 2 weeks later and found out the implant did not take, my hCG levels were less than 1-and those values should double every day (and be like 5-10 value). The only positive in this sad time is that a failed implant is a failed implant, because it did not take-it does not count as a miscarriage.

Me and my partner decided to remove me from the equation as far as carrying goes. I am going to recover, he's going to work and save money. I am 32 years old this year, by the time we get to 35-40...we are in trouble if we don't have results - as far as my body goes. So if I can get one more Egg Retrieval done after rest by 35, and let someone else carry it-we can still make this happen. It is pretty amazing how many years can go by trying to do this thing though, this is year 5/6 for us so far.

Ya know it's a funny thing, the longer I do this-the less self conscious I feel about it? Like before I would be all worried for anyone to know I was too busy because of an injection, and while I boldly share this via text-I still kind of keep it private ish? It is funny how things change in such a short amount of time, just a couple of years ago I was terrified to even jump in.

[teknokusak]

Kanserin Erken Tespiti: Aneuploid Hücrelerin Rolü ve Yeni Bulgular

Kanserin Erken Tespiti: Yeni Bulguların Anlamı Kanser tedavi yöntemleri sürekli olarak gelişmeye devam ederken, bu hastalığın erken aşamalarda tespiti de büyük bir önem arz ediyor. Vücutta kanser hücrelerinin varlığını tespit etmek için doktorların başvurduğu yöntemlerden biri, kanser belirteçleri olarak bilinen biyomarkerlerdir. Bu belirteçler, kanserin varlığına dair ipuçları sunar. Ancak, son…

[innonurse]

AI-based system BELA enhances IVF embryo assessment with time-lapse imaging and maternal age analysis

- By InnoNurse Staff -

A new AI-based system called BELA, developed by researchers at Weill Cornell Medicine, can assess the chromosomal status of IVF embryos using time-lapse video images and maternal age.

BELA improves on prior AI methods by generating an objective embryo quality score, predicting whether an embryo has a normal (euploid) or abnormal (aneuploid) number of chromosomes without relying on embryologists’ subjective evaluations.

The system was trained on data from nearly 2,000 embryos and showed higher accuracy than earlier models when tested on external datasets.

If validated through clinical trials, BELA could enhance the IVF process, reduce the need for invasive genetic testing, and improve accessibility to IVF treatments globally. The team envisions BELA being used for other embryo assessments in the future.

Header image: An AI-based tool can help embryologists evaluate both embryo quality and ploidy status, offering a thorough assessment of the embryo. Credit: Suraj Rajendran.

Read more at Weill Cornell Medicine

[healthcareresearchupdates]

Preimplantation Genetic Testing Market: Growth Drivers and Opportunities

As per the recently published report by MarketsandMarkets™, The report  “Preimplantation Genetic Testing Market by Procedure (Diagnosis, Screening), Technology (NGS, PCR, FISH, CGH, SNP), Product (Consumable, Instrument), Application (Aneuploid, HLA Typing), Type of Cycle, End User, and Region – Global Forecast to 2028″, is projected to reach USD 1.2 billion by 2028 from USD 0.7 billion in 2023,…

[yeast-papers]

Premature aging in aneuploid yeast is caused in part by aneuploidy-induced defects in Ribosome Quality Control

BioRxiv: http://dlvr.it/T8grz8

[ribosome-papers]

Premature aging in aneuploid yeast is caused in part by aneuploidy-induced defects in Ribosome Quality Control

bioRxiv: http://dlvr.it/T8grFs

[cytgen]

Abstract Worldwide, endometrial cancer is one of the most frequently diagnosed malignancies in women and a notable cause of death. The aim of this study was to perform image cytometric DNA ploidy analysis on a prospective material of endometrial adenocarcinomas in order to determine potential correlation between ploidy status and their histological features. The analysis was carried out in fresh tissue samples resected by implementing complete hysterectomy in a series of patients (n = 126). We found that ploidy status using image cytometry correlate with histologic type, grade and stage in endometrial cancer and aneuploid tumor samples are associated with aggressive phenotype statistics. Furthermore, DNA ploidy should be used as a reliable and applicable prognostic marker in the routine clinical practice.

Keywords: DNA ploidy Image cytometry diploid aneuploidy endometrial carcinoma endometrial cancer

[healthcareresearchupdates]

Preimplantation Genetic Testing Market: Growth Drivers and Opportunities

As per the recently published report by MarketsandMarkets™, The report  “Preimplantation Genetic Testing Market by Procedure (Diagnosis, Screening), Technology (NGS, PCR, FISH, CGH, SNP), Product (Consumable, Instrument), Application (Aneuploid, HLA Typing), Type of Cycle, End User, and Region – Global Forecast to 2028″, is projected to reach USD 1.2 billion by 2028 from USD 0.7 billion in 2023,…

[freedomainnames]

aneuploid-cul-de-sac.org

[dallasfertilitycenter]

Fresh vs. Frozen Embryo Transfer: Exploring the Differences

There are many decisions to make on your journey to becoming a parent. Which infertility clinic is a good fit? What treatments are you willing to undergo? Is it better to transfer fresh or frozen embryos for IVF (In Vitro Fertilization)? Luckily, your treatment team can help guide you to the choices that are right for you, and Dr. Walid Saleh of SIRM Dallas in Dallas, Texas can give you some insight into the pros and cons of fresh versus frozen embryo transfer below.

A Fresh Perspective

All embryos start out fresh. In a woman’s initial IVF cycle, she takes medication to first “quiet” the ovaries followed by medication to stimulate them to produce extra eggs. After the eggs have been surgically retrieved, they are fertilized. The resulting embryos grow in a special incubator until they are ready to be transferred to the uterus. In most cases, one embryo is selected for transfer and the remaining embryos are frozen. Fresh cycles were, for decades, the gold standard…until the last few years when rapid advancements in freezing and cryopreservation methods improved the outcome of frozen embryo transfers.

Back in the Day

First, a little history on frozen embryo transfer (FET). Embryos were initially frozen slowly which caused intracellular ice to form; unfortunately, the ice often damaged the embryos. Many damaged embryos didn’t survive, and those that did had very low potential for successful implantation. However, in the last several years we’ve seen a dramatic increase in the quality of frozen embryos due to the introduction of vitrification. Vitrification is an ultra-rapid freezing method that freezes the embryo approximately 60,000 times faster than the older method of freezing. This process takes ice formation out of the equation, resulting in a significant increase in viable embryos with successful implantation and pregnancy potential equal to fresh embryos.

If you think frozen embryo transfers may be right for you, schedule an appointment at SIRM Dallas in Dallas, Texas to learn more.

Timing is Everything

Another reason we see markedly higher FET success rates now than several years ago is that the timing of when the embryo is frozen has changed. It’s been shown that freezing blastocysts (embryo at day 5-6) has a better outcome than freezing early-cleaved embryos (day 2-3). In many cases embryos that don’t successfully develop to the expanded blastocyst stage are aneuploid (chromosomally compromised) and don’t result in healthy babies. An additional benefit of frozen embryo transfers using expanded blastocysts is that fewer embryos – because we’re able to determine which are most viable – need to be transferred. This means we can minimize the chance of a multiple pregnancy, along with the inherent risks to both mom and baby.

Fresh or Frozen?

At this point, studies show that successful outcomes using frozen embryo transfers are about equal to those of fresh embryo transfers. The slight edge that fresh embryo transfer may have is that, typically, the best embryo is selected for the first transfer. However, there are some key benefits that make FET appealing:

Because you underwent ovarian stimulation and egg retrieval for your fresh cycle, you won’t have to go through it again. The medications necessary to prepare your body for FET have fewer potential side effects and take less of a toll on your body.

Many patients report that FET cycles are not nearly as stressful as fresh cycles since they already know they have viable embryos.

It’s easier to schedule and plan for FET cycles than fresh cycles.

If you’d like to learn more about how fresh and/or frozen embryo transfers and IVF may benefit you, schedule an appointment at SIRM Dallas in Dallas, Texas to meet with Dr. Saleh and discuss your options.

[sapropel]

Your aneuploidous boyfriend is giving me a huge throbbing boner in my kingdom heart

[lisajkent86]

The Potential of Artificial Intelligence in Improving IVF Success Rates

A recent study published in The Lancet Digital Health by Josue Barnes and colleagues introduced an innovative AI-driven method called STORK-A. Reportedly, this non-invasive technique can predict the ploidy status of embryos, thereby making it easy to select healthy blastocysts for implantation.

But before we go further exploring the potential of this method, one must know that STORK-A will never be used as a replacement option for conventional preimplantation genetic testing for aneuploid status (PGT-A). Instead, it would strictly act as a complementary way to assist clinicians in their decision-making. Furthermore, STORK-A is still largely a proof-of-concept approach, backed by one of the largest studies of its kind. Thus, any external validation will only demonstrate the integrative potential of AI under pre-decided clinical settings for embryo selection.

AI-assisted embryo selection- Diversity and Inclusivity Holding The Key

Inclusive participation across multiple trials and studies is vital to ensuring generalizability and equitable benefits. A systematic review and meta-analysis from 2022 have already revealed the inherent disparities in IVF outcomes. especially with black women experiencing higher rates of spontaneous abortions and lower live birth rates compared to white females. Although recent studies conducted by Hajirasouliha and colleagues offered validation across multiple populations, the disparity of race or ethnicity data of participants still remains a vital aspect for all forms of future research.

Undoubtedly, the integration of AI in embryo selection for IVF holds untold potential for the future. However, significant challenges lie ahead that will call for repeated reassuring on multiple grounds before such methods can become a part of routine clinical decisions. Sure, large-scale RCTs encompassing diverse populations with external validation have a way out, but the current scenario calls for deep evaluation of externally validated AI approaches to establish befitting clinical value.

For more local news please follow our blog.

Read More: Artificial Intelligence in Improving IVF Success Rates

[theaussieway]

The Potential of Artificial Intelligence in Improving IVF Success Rates

A recent study published in The Lancet Digital Health by Josue Barnes and colleagues introduced an innovative AI-driven method called STORK-A. Reportedly, this non-invasive technique can predict the ploidy status of embryos, thereby making it easy to select healthy blastocysts for implantation.

But before we go further exploring the potential of this method, one must know that STORK-A will never be used as a replacement option for conventional preimplantation genetic testing for aneuploid status (PGT-A). Instead, it would strictly act as a complementary way to assist clinicians in their decision-making. Furthermore, STORK-A is still largely a proof-of-concept approach, backed by one of the largest studies of its kind. Thus, any external validation will only demonstrate the integrative potential of AI under pre-decided clinical settings for embryo selection.

AI-assisted embryo selection- Diversity and Inclusivity Holding The Key

Inclusive participation across multiple trials and studies is vital to ensuring generalizability and equitable benefits. A systematic review and meta-analysis from 2022 have already revealed the inherent disparities in IVF outcomes. especially with black women experiencing higher rates of spontaneous abortions and lower live birth rates compared to white females. Although recent studies conducted by Hajirasouliha and colleagues offered validation across multiple populations, the disparity of race or ethnicity data of participants still remains a vital aspect for all forms of future research.

Undoubtedly, the integration of AI in embryo selection for IVF holds untold potential for the future. However, significant challenges lie ahead that will call for repeated reassuring on multiple grounds before such methods can become a part of routine clinical decisions. Sure, large-scale RCTs encompassing diverse populations with external validation have a way out, but the current scenario calls for deep evaluation of externally validated AI approaches to establish befitting clinical value.

For more local news please follow our blog.

Read More: Artificial Intelligence in Improving IVF Success Rates

[heavenkills]

-kisses her on the fucking lips-

grell’s    first    reaction    is    one    of    surprise    ———–    moira    can    SEE    her    ?    !    that    can’t    be    good.    but    then    she    moans,    leans    up    to    return    her    kiss.    it’s    heated    and    passionate,    just    like    she    likes    it,    and    for    a    moment    grell    feels    like    she’s    dreaming.    it’s    not    very    often    a    hot    woman    standing    over    a    corpse    grabs    you,    pulls    you    close,    and    snogs    you    so    fervently    that    you    feel    like    your    legs    might    give    out,    especially    not    when    you    are    DEATH.    she’s    living    a    charmed    life,    and    she    wouldn’t    give    it    up    for    anything    in    the    world.

eventually,    moira    has    to    pull    away    for    breath,    and    grell    smirks    like    the    cat    that    caught    the    canary.    god,    she’s    good    looking,    isn’t    she    ?    ❝    you’ve    got    some    GUTS,    don’t    you,    darling    ?    oh,    i    like    that.    ❞