#RDRP

[arkethamz]

corleone art dump bc i simply cant draw anything else :) /hj

[cowgworl]

i love rp sometimes lmfaooo

[jackofhearts-odb]

A piece I did of Vulture sometime last week to try and get his official look down. Can you believe this was done entirely with 1 brush?

[loveless-lotus]

Some Western Norse Cowboy shenanigans

[firehouselight]

Heeyyyyyyy so uhhh long story short a couple months ago I got REALLY into watching streamers rp in a red dead redemption server called Goldrush (and now sometimes Ranch too) and it literally pulled me out of a 4-year-long burnout and some deep depression so EXPECT TO SEE MORE OF THESE COWBOYS. We’ll say this is RDRP dump #1.

Characters are played on twitch by starboychrizz, Granny, IndoorScream, and Hayley_Corinne, and I love them lots 💚

[mephibon]

rdrp was already full of shitty people to begin with so i’m not sure why i’m surprised to see Real People cosplaying indigenous folk (band unspecified or straight up conjured) on rp servers😭

it’s obviously super frustrating to bear witness to, but the accents they put on are truly the cherry on top of the stinking pile of shit. then again, saying things like “the sioux and the apache nations are the same” (paraphrasing) when they’re regions apart is more embarrassing on their end…overall it’s not strange to HAVE an indigenous oc if you know wtf you’re talking about, but there’s a huge fucking difference between written and spoken rp.

sticking a bunch of eagle and owl feathers in your character model’s hair, or a straight up full headdress on em, is definitely not the way to go about being respectful..this shouldn’t even have to be said☹️

[jjjordiiii]

Back when I used to rp in Red Dead Online 2 of the dumbest rules were: you couldn't have 2 guns on your back specifically because it'd be too heavy (it would've been ~18lbs) and you couldn't have gold guns, not because it looked bad but because gold melted faster. It completely ignored that the guns would be gold plated and not made of gold.

Don't roleplay in rdo btw, half of roleplayers are Nazis or dickheads. Legit had a guy seem like the chillest dude ever and one day he made his pfp Andrew Tate and ranted about how much he hated trans people. Toxic community that lacks creativity and always bitches and whines when they get killed.

Also they can just be cringe in a way I don't fw. "My left revolver has a pearl grip and I use it when I kill for a good cause and my right revolver has an ebony grip and I use it when killing for bad reasons". That was a real statement I heard.

Back onto the "lacking creativity" part every single server would just be the same shit with similar characters. Rich guy who is corrupt, outlaw but idealistic, outlaw but bad, determined lawman, corrupt lawman, lawyer, some type of mafia which is either the Italian-American mob or the Irish mob, racist, guy who hates racists, and woman who gets taught how to use a gun.

Tldr: I hate roleplaying in rdr2 and the role playing community is racist and transphobic and overall sucks mad shit.

[lanciet]

I'm a liar, I missed hearing malton yap

[jppres]

In silico analysis of anti-dengue activity of faloak (Sterculia quadrifida R. Br) stem bark compounds

Image: Wikimedia Article published in J. Pharm. Pharmacogn. Res., vol. 10, no. 6, pp. 1006-1014, November-December 2022. DOI: https://doi.org/10.56499/jppres22.1445_10.6.1006 Audrey G. Riwu1, Jusak Nugraha2, Djoko A. Purwanto3*, Erwin A. Triyono4 1Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo 47, 60131, Surabaya,…

[arkethamz]

Antonio redraw n more lighting/shading practice from last month :3

[rnomics]

Microorganisms, Vol. 12, Pages 2: Assessing Genomic Mutations in SARS-CoV-2: Potential Resistance to Antiviral Drugs in Viral Populations from Untreated COVID-19 Patients

Naturally occurring SARS-CoV-2 variants mutated in genomic regions targeted by antiviral drugs have not been extensively studied. This study investigated the potential of the #RNA-dependent #RNA polymerase (RdRp) complex subunits and non-structural protein (Nsp)5 of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) to accumulate natural mutations that could affect the efficacy of antiviral drugs. To this aim, SARS-CoV-2 genomic sequences isolated from 4155 drug-naive individuals from southern Italy were analyzed using the Illumina MiSeq platform. Sequencing of the 4155 samples showed the following viral variant distribution: 71.2% Delta, 22.2% Omicron, and 6.4% Alpha. In the Nsp12 sequences, we found 84 amino acid substitutions. The most common one was P323L, detected in 3777/4155 (91%) samples, with 2906/3777 (69.9%) also showing the G671S substitution in combination. Additionally, we identified 28, 14, and 24 different amino acid substitutions in the Nsp5, Nsp7, and Nsp8 genomic regions, respectively. Of note, the V186F and A191V substitutions, affecting residues adjacent to the active site of Nsp5 (the target of the antiviral drug Paxlovid), were found in 157/4155 (3.8%) and 3/4155 (0.07%) samples, respectively. In conclusion, the RdRp complex subunits and the Nsp5 genomic region exhibit susceptibility to accumulating natural mutations. This susceptibility poses a potential risk to the efficacy of antiviral drugs, as these mutations may compromise the drug ability to inhibit viral replication https://www.mdpi.com/2076-2607/12/1/2?utm_source=dlvr.it&utm_medium=tumblr

[jackofhearts-odb]

Double sided charm design of Vulture that I'm eventually going to get made into keychains. Was a lot of fun to do!

[screenshots123]

📆 2020 April 10 ✍️ Laurel Oldach 📰 Slipping past the proofreader

When the virus behind SARS was identified, one of the many experimental treatments clinicians tried using was a molecule called ribavirin. At the time, ribavirin was the first-line drug for many RNA viruses.

Ribavirin targets a viral protein called the RNA-dependent RNA polymerase, or RdRp, which is responsible for replicating the coronavirus genome.

“We were surprised that ribavirin was pretty toxic and not very effective (against) coronaviruses,” Canard said.

As researchers in the field came to understand the coronavirus RdRp better, they found that the polymerase by itself could not explain the disconnect. Virologist François Ferron, a staff scientist at CNRS, has worked on coronavirus replication since the SARS outbreak. “The viral RNA polymerase is quite loose, meaning it has a tendency to make a lot of mistakes,” he said. “Maybe a little bit more than the regular (cellular) RNA polymerase.”

This led researchers to suspect that coronaviruses might have some way of recognizing and correcting errors.

“There was a speculation that (coronaviruses) might encode a proofreading function that would allow them to stabilize a big genome, and there was a predicted place in the genome where that might occur,” Denison explained. “That really led us to try the genetic experiments.”

In 2007, his group found that in viruses lacking the protein encoded at that same location, a protein called nsp14, coronaviruses from a mouse model virus accumulated mutations at a rate similar to other RNA viruses. And strains of the virus without nsp14, they found, were sensitive to ribavirin.

Following up on that work, the French group zeroed in on how nsp14 worked in a test tube. “We didn’t work on the virus, like Mark Denison was beautifully doing,” said Canard. “We just concentrated on that enzyme … and we found out that it could actually excise ribavirin.”

The CNRS team published an enzymology study that confirmed that nsp14 can identify and remove mismatches between bases at the end of a growing copy of viral RNA; in 2018 they followed up with confirmation that when ribavirin is incorporated in a growing RNA strand, nsp14 protein can scoop it out of the stalled strand, letting replication resume.

[firehouselight]

RDRP Comic #12: Aisle

Script taken from IndoorScream and starboychrizz on twitch

[darkmaga-returns]

On November 11, 2024, an article was published online in Business Wire pertaining to the launch of a clinical trial NCT06602531 intended to test “Safety and Immunogenicity Study of Self-Amplifying RNA Pandemic Influenza Vaccine in Adults.” 

Question: Why is the word “Pandemic” in the title? 

The article provides information on the ARCT-2304 product which is a “sa-mRNA vaccine candidate formulated within a lipid nanoparticle (LNP).”

So. Many. Questions. For example, beyond the use of the word pandemic in the study title, why is this product being called a vaccine? Why is a new version of a product riddled with unresolved compendial standard issues being trialed?

ARCT-2304 is a gene-therapy-based prodrug that uses self-amplifying RNA technology (specifically, the RNA-dependent RNA polymerase (RdRP) gene, which allows it to replicate autonomously) that originates from an Alphavirus. Just so you know, this makes these products genetically-modified organisms (GMOs) and this is because of the fact that the coding template is a modified Alphavirus genome with the virus sub-genomic bits spiked out and the foreign flu genes ‘spiked in.’ The genetic material is capable of reproduction. The following slide shows how they did this for the Covid-19 version (KOSTAIVE® (ARCT-154) Monovalent: JN.1).