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leedsomics · 9 months ago
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Deep plasma proteomics with data-independent acquisition: A fastlane towards biomarkers identification.
Plasma proteomic is a precious tool in human disease research, but requires extensive sample preparation in order to perform in-depth analysis and biomarker discovery using traditional Data-Dependent Acquisition (DDA). Here, we highlight the efficacy of combining moderate plasma prefractionation and Data-Independent Acquisition (DIA) to significantly improve proteome coverage and depth, while remaining cost- and time-efficient. Using human plasma collected from a 20-patient COVID-19 cohort, our method utilises commonly available solutions for depletion, sample preparation, and fractionation, followed by 3 LC-MS/MS injections for a 360-minutes DIA run time. DIA-NN software was then used for precursor identification, and the QFeatures R package was used for protein aggregation. We detect 1,321 proteins on average per patient, and 2,031 unique proteins across the cohort. Filtering precursors present in under 25% of patients, we still detect 1,230 average proteins and 1,590 unique proteins, indicating robust protein identification. Differential analysis further demonstrates the applicability of this method for plasma proteomic research and clinical biomarker identification. In summary, this study introduces a streamlined, cost- and time-effective approach to deep plasma proteome analysis, expanding its utility beyond classical research environments and enabling larger-scale multi-omics investigations in clinical settings. http://dlvr.it/T3Jtby
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ricos1161 · 7 years ago
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Monger Culture: Sex tourism and HIV in Costa Rica
To commemorate the World AIDS Day, today December 1, we feature the article “Monger Culture: Sex tourism and HIV in Costa Rica” by Jacobo Schifter, published on Wall Street International , June 20, 2017.
(Wsimag.com) When we think of HIV we have certain populations in mind. We hear about its ravages on young men and women; on the gay and transgender populations; on the homeless and the…
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qmediaworld · 7 years ago
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Monger Culture: Sex tourism and HIV in Costa Rica
To commemorate the World AIDS Day, today December 1, we feature the article “Monger Culture: Sex tourism and HIV in Costa Rica” by Jacobo Schifter, published on Wall Street International , June 20, 2017.
(Wsimag.com) When we think of HIV we have certain populations in mind. We hear about its ravages on young men and women; on the gay and transgender populations; on the homeless and the…
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ricos1161 · 7 years ago
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Ivanka and The Fugitive From Panama
(REUTERS) PANAMA CITY/TORONTO – In the spring of 2007, a succession of foreigners, many from Russia, arrived at Panama City airport to be greeted by a chauffeur who whisked them off in a white Cadillac with a Donald Trump logo on the side.
SNAPPED: Ivanka Trump and Alexandre Ventura Nogueira at Trump Tower in New York in 2006, and Donald Trump with Nogueira at Mar-a-Lago in 2007. The photographs…
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qmediaworld · 7 years ago
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Ivanka and The Fugitive From Panama
(REUTERS) PANAMA CITY/TORONTO – In the spring of 2007, a succession of foreigners, many from Russia, arrived at Panama City airport to be greeted by a chauffeur who whisked them off in a white Cadillac with a Donald Trump logo on the side.
SNAPPED: Ivanka Trump and Alexandre Ventura Nogueira at Trump Tower in New York in 2006, and Donald Trump with Nogueira at Mar-a-Lago in 2007. The photographs…
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ricos1161 · 7 years ago
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Why Story of Russia's Meddling in 2018 Mexican Vote Causes Laughter
Why Story of Russia’s Meddling in 2018 Mexican Vote Causes Laughter
The rumors of Russia’s alleged intention to interfere in the 2018 Mexican presidential elections are completely groundless and ridiculous, Carlos Figueroa Ibarra, researcher at the Benemérita Universidad Autónoma de Puebla, told Sputnik, dropping a hint as to who could be behind the rumors circulated by the Mexican press.
Mexican press on alert to the almighty Putin, however, Mexico has no…
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qmediaworld · 7 years ago
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Why Story of Russia's Meddling in 2018 Mexican Vote Causes Laughter
Why Story of Russia’s Meddling in 2018 Mexican Vote Causes Laughter
The rumors of Russia’s alleged intention to interfere in the 2018 Mexican presidential elections are completely groundless and ridiculous, Carlos Figueroa Ibarra, researcher at the Benemérita Universidad Autónoma de Puebla, told Sputnik, dropping a hint as to who could be behind the rumors circulated by the Mexican press.
Mexican press on alert to the almighty Putin, however, Mexico has no…
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ricos1161 · 7 years ago
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Three Families Receive A Quarter Of Government Spending On Rent
Five corporations (Sociedades Anominas or S.A.s’), controlled by three families, receive 24% of the ¢1.589 billion colones that 15 government agencies pay monthly in the rental of 303 properties.
An investigative report by Amelia Ruedareveals the companies, controlled by the families Marín Raventós, Saavedra Raventós, and Escobar Pardo, invoice the government ¢382 million colones (US$682,000…
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qmediaworld · 7 years ago
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Three Families Receive A Quarter Of Government Spending On Rent
Five corporations (Sociedades Anominas or S.A.s’), controlled by three families, receive 24% of the ¢1.589 billion colones that 15 government agencies pay monthly in the rental of 303 properties.
An investigative report by Amelia Ruedareveals the companies, controlled by the families Marín Raventós, Saavedra Raventós, and Escobar Pardo, invoice the government ¢382 million colones (US$682,000…
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ricos1161 · 7 years ago
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NAFTA Unlikely To Hurt Mexico-U.S. Security Ties, But Election Might
(Reuters) – Mexico’s threats to weaken U.S. narcotics and migration co-operation if NAFTA dies are pure bluster, Mexican and U.S. officials say, with next year’s election posing a far greater challenge to future collaboration.
Mexican officials have threatened to strike back if U.S. President Donald Trump kills the North American Free Trade Agreement (NAFTA), warning they could relax controls on…
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qmediaworld · 7 years ago
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NAFTA Unlikely To Hurt Mexico-U.S. Security Ties, But Election Might
(Reuters) – Mexico’s threats to weaken U.S. narcotics and migration co-operation if NAFTA dies are pure bluster, Mexican and U.S. officials say, with next year’s election posing a far greater challenge to future collaboration.
Mexican officials have threatened to strike back if U.S. President Donald Trump kills the North American Free Trade Agreement (NAFTA), warning they could relax controls on…
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ricos1161 · 7 years ago
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Tipping Points Over Crossed: Emergency in New Delhi
The following is a post on Facebook by Costa Rica Ambassador to India, Marieal Cruz Alavarez, appointed to the post last May and in the past week having to leave the Indian capital after she developed a respiratory illness.
From Facebook
She is the first among the Delhi-based diplomatic community to complain about the New Delhi’s unbreathable air.
Related: Costa Rica Ambassador Says ‘India I Love…
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qmediaworld · 7 years ago
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Tipping Points Over Crossed: Emergency in New Delhi
The following is a post on Facebook by Costa Rica Ambassador to India, Marieal Cruz Alavarez, appointed to the post last May and in the past week having to leave the Indian capital after she developed a respiratory illness.
From Facebook
She is the first among the Delhi-based diplomatic community to complain about the New Delhi’s unbreathable air.
Related: Costa Rica Ambassador Says ‘India I Love…
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ricos1161 · 7 years ago
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Mobility in Latin America: Doing better than parents but not peers
Mobility in Latin America: Doing better than parents but not peers
In a promising sign of economic mobility across generations, access to education has grown leaps and bounds in Latin America and the Caribbean in recent decades, more so, in fact, than any other region.
From Weforum.org
Today more children are going to school for a longer time – and are therefore more educated and prone to have better incomes – than their parents before them.
Yet this apparent…
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qmediaworld · 7 years ago
Text
Mobility in Latin America: Doing better than parents but not peers
Mobility in Latin America: Doing better than parents but not peers
In a promising sign of economic mobility across generations, access to education has grown leaps and bounds in Latin America and the Caribbean in recent decades, more so, in fact, than any other region.
From Weforum.org
Today more children are going to school for a longer time – and are therefore more educated and prone to have better incomes – than their parents before them.
Yet this apparent…
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ricos1161 · 7 years ago
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Is daylight saving time worth the trouble? Research says no
Is daylight saving time worth the trouble? Research says no
Daylight saving time (DST) ends Sunday, when clocks in many parts of the United States, Canada, Europe and the world, will turn back one hour.
While Costa Rica does not observe DST, we are affected by it, directly and indirectly, like watching U.S. television or calling family and friends up north.
There’s a lot of confusion. But all will be right again in the world tomorrow.
Though many American…
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