#Fabien Tarlet

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[tomub]

Fabien Tarlet

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Tweeted

Nombres de mort depuis 2000 en France à cause: - du terrorisme: 281 - de la chasse: 366 - de la violence conjugale: 2160

— Fabien Tarlet (@FTarlet) November 20, 2019

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Lung stereotactic arc therapy in mice: development of radiation pneumopathy and influence of HIF-1α endothelial deletion

Publication date: Available online 29 January 2019

Source: International Journal of Radiation Oncology*Biology*Physics

Author(s): Jérémy Lavigne, Alexandra Suissa, Nicolas Verger, Morgane Dos Santos, Mohamedamine Benadjaoud, Laurence Mille-Hamard, Iman Momken, Frédéric Soysouvanh, Valérie Buard, Olivier Guipaud, Vincent Paget, Georges Tarlet, Fabien Milliat, Agnès François

Abstract

Purpose

Stereotactic body radiation therapy (SBRT) offers good lung local tumor control by the administration of a high dose per fraction in small volumes. SBRT preclinical modeling is now possible, and our aim was to develop a model of focal irradiation of the mouse lung and to investigate the impact of conditional HIF-1α deletion in the endothelium on radiation-induced tissue damage.

Methods and materials

The Small Animal Radiation Research Platform (SARRP) was used to create a mouse model of focal irradiation of the lung using arc therapy. HIF-1α conditional deletion was obtained by crossing mice expressing Cre Recombinase under the endothelial promoter VE-cadherin (VECad-Cre+/+ mice (1)) with HIF-1α floxed mice.

Results

Lung stereotactic arc-therapy allows thoracic wall sparing and long-term studies. However, isodose curves showed that neighboring organs received significant doses of radiation, as revealed by ipsilateral lung acute red hepatization and major gene expression level modifications. Conditional HIF-1α deletion reduced acute lung edema and tended to diminish neutrophil infiltrate, but had no impact on long-term global tissue damage.

Conclusions

Arc therapy for focal high-dose irradiation of mouse lung is an efficient model for long-term studies. However, it is possible that irradiation may have a strong impact on the structure and function of neighboring organs which have to be taken into account. HIF-1α conditional deletion has no beneficial impact on lung damage in this irradiation schedule.

http://bit.ly/2B4O8pF

[cinqpetitessecondes]

Tweeted

Mélenchon sera donc jugé par une magistrate qui s'affiche fièrement sur la page "Ensemble avec Emmanuel Macron". Mais ce n'est bien évidemment pas du tout un procès politique.https://t.co/jXQBFDHuoY

— Fabien Tarlet (@FTarlet) September 17, 2019

[cancersfakianakis1]

Conditional Plasminogen Activator Inhibitor Type 1 Deletion in the Endothelial Compartment Has No Beneficial Effect on Radiation-Induced Whole-Lung Damage in Mice

Publication date: 15 November 2017 Source:International Journal of Radiation Oncology*Biology*Physics, Volume 99, Issue 4 Author(s): Jérémy Lavigne, Frédéric Soysouvanh, Valérie Buard, Georges Tarlet, Olivier Guipaud, Vincent Paget, Fabien Milliat, Agnès François PurposeTo investigate whether the endothelial pool of plasminogen activator inhibitor type 1 (PAI-1) plays a role in the development of radiation-induced lung damage, as previously demonstrated in the intestine.Methods and MaterialsHuman lung microvascular endothelial cells were exposed to 10 Gy irradiation so as to study their ability to acquire an “activated” phenotype. Mice in which the Cre-Lox strategy was used to produce PAI-1 deletion specifically in the endothelial compartment were exposed to 17 Gy whole-thorax irradiation and followed up for 2, 13, and 23 weeks after irradiation.ResultsHuman lung microvascular endothelial cells had an activated phenotype after radiation exposure, overexpressed PAI-1, and underwent endothelial-to-mesenchymal transition. In mice, knockout of PAI-1 in the endothelium had no beneficial effect on radiation-induced lung damage and showed a tendency to worsen acute lesions.ConclusionsAs opposed to the intestine, the endothelial pool of PAI-1 does not play a determinant role in the development of radiation-induced lung damage. The therapeutic value of PAI-1 inhibition in lung radiation injury may be associated with other types of cells. http://ift.tt/2h2fG53