#Dry Bath Incubator Manufacturers in India

[midseo]

Low Temperature Bath, Hot Air Oven, Humidity Control Oven, India

Aditi Associate : Manufacturer and Exporter of Low Temperature Bath, Hot Air Oven, Humidity Control Oven, Laboratory Autoclave, Mumbai, India.

Laboratory Equipments, Dehumidifiers, Weighing Scales, Low Temperature Bath, Hot Air Oven, Laboratory Autoclave, Humidity Control Oven, Stainless Steel Autoclave, Hot Air Oven, Tray Dryers, Bod Incubator, Bacteriological Incubator, Stability Chamber, Humidity Oven, Laminar Air Flow, Biosafety Cabinet, Muffle Furnace, Orbital Shaker Incubator, Rotary Shaker, Deep Freezer, Fume Chamber, Autoclave, High Temperature Industrial Furnace, Viscometer Water Bath, Vacuum Oven, Clean Room Equipment, Blood Bank Refrigerator, Spectrophotometer, Ultrasonic Cleaners, Uv Cabinet, Dry Bath, Uv Inspection Cabinet, Vacuum Pumps, Colony Counter, Ph Meter, Conductivity Meter, Probe Type Ultrasonic Cleaner, Mumbai, India

www.aditiassociate.in

[promotiononline-blog]

Best cooling baths manufacturer and supplier in Delhi India.

We are the best cooling baths manufacturer and supplier in Delhi India. Roch Mechatronics Inc. Dry Bath Incubator is a microprocessor-controlled product using the advanced thermoelectric technique. With this technique, more stable and accurate temperature control is achieved.

For more information,

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[omc-juniperpublishers]

Phytochemical Profiling and Antifungal Activity m of Essential Oil and Rhizome Extracts of Curcuma Amada Roxb-JuniperPublishers

Journal of Chemistry-JuniperPublishers

Abstract

Curcuma amada (Zingiberaceae), commonly used as spice, and is also associated with multiple health benefits. In the present study, petroleum ether, cyclohexane, ethyl acetate, chloroform, acetone and methanol extracts of dried rhizome of C. amada prepared by solvent extraction were analyzed for quantitative and qualitative phytochemical profile and antifungal assay. In vitro antifungal activity of all extracts was determined by using agar well diffusion method against four fungi (Rhizoctonia solani, Sclerotium rolfsii, Collectotricum falcatum and Sclerotenia solani). The essential oil and extracts were found to active against the tested fungi in dose dependent manner by inhibiting the fungul mycelia growth. The essential oil of C. amada exhibited higher activity in comparison to extracts against all the fungi. Maximum inhibition of fungul growth was recorded for R. solani (80.93%) and S. solani (80.90%).

Keywords: Curcuma amda; Extracts; Essential oil; Phytochemicals; Antifungal activity

Research Article

The genus Curcuma belonging to the family Zingiberaceae has a widespread occurrence in the tropical Asia and Australia. This genus, comprises of more than 80 species of rhizomatous herbs including Curcuma amada, Curcuma longa, Curcuma zedoaria, Curcuma aromatica, is widely used in traditional systems of medicines such as Ayurveda, Siddha, Unani, Homeopathy and Naturopathy. In India, it is cultivated in innumerable agro- ecological situations right from the coastal areas to elevations as high as 1880m in the tropics and the sub-tropics of the country [1-3]. Curcuma amada Roxb. It is commonly known as Amada or 'Amahaldi' or 'mango ginger' due to the raw mango-like aroma of the rhizome. Curcuma amada Roxb.

Is a rhizomatous aromatic herb with a leafy tuft and 6090cm in height? Leaves are long, petiolate, oblonglanceolate, tapering at both ends, glabrous and green on both sides. Flowers are white or pale yellow, arranged in spikes in the centre of tuft of the leaves. Lip is semi-elliptic, yellow, 3-lobbed with the mid lobe emarginated [4]. Curcuma amada possess antifungal, antiinflammatory, anticancer and anti hyperglyceridemic properties [5-7]. Rhizomes of Curcuma amada Roxb. Used for the manufacture of oleoresin and essential oil [8]. Its rhizomes essential oil containing β-myrcene, β-pinene, α-pinene, ocimene, ar-curcumene, linalool, linalyl acetate, camphor and safrole [9,10]. Based on the above facts it is imperative to investigate the qualitative phytochemical variations and antifungal activity in various extracts and essential oil.

Material and Methods

Plant material

Air dried rhizomes of C. amada were collected from the local agricultural field of District Udham singh nagar Uttarakhand, India and identified by Dr. D.S. Rawat (plant taxonomist), G.B.Pant University of Agriculture and Technology, Pantnagar. Rhizomes were washed thoroughly to remove adhering material and shade dried at room temperature and was further ground by means of an electrical blender to fine powder.

Isolation of the essential oil

The fresh rhizomes were hydro distilled for using a Clevenger type apparatus for 8h. The oil was extracted with the help of dichloromethane followed by drying over anhydrous Na2SO4. The yield of oil was found about 0.52% (w/v).

Preparation of plant extracts

Plant extracts were prepared in six different organic solvents (Petroleum ether, cyclohexane, ethyl acetate, chloroform, acetone and methanol) using solvent extraction [11]. Rhizome powder (300 g) was extracted in 150 ml of each solvent separately using Soxhlet extractor over water bath for 8h. The extracts were concentrated using vacuum rotatory evaporator at 45±5°C.

Phytochemical screening

Qualitative and quantitative analysis bioassay for total phenol [12], flavanols and orthodihydroxy phenols [13], phytochemical screening of all the six extracts for presence of alkaloids, tannin, anthraquinone, glycosides, reducing sugar, saponins, flavonoids, terpenoids, caumarine, emodins, anthocyanin, betacyanin was carried out according to standard method reported earlier [14-16].

Antifungal activity

The antifungal activity of different solvent extracts was determined by agar well diffusion method [17,18]. Four phytopathogenic fungi, Rhizoctonia solani, Sclerotium rolfsii, Colletotricum falcatum and Sclerotenia solani were maintained and grown on potato dextrose agar medium in order to study antifungal activity of essential oil and various extracts having different polarity.

Sterilized petri plates of 90 mm diameter were used for pouring of medium. In each petri plate about 20 ml sterilized melted medium was aseptically poured near burner flame in a sterilized laminar air flow chamber The medium in the plates were centrally inoculated by placing a 5 mm mycelial disc which was cut from the margin of 5 days old culture of the test fungus. Sterilized four filter paper disc were placed in a sterilized petri plates and different concentration of the extracts were added with the help of sterilized micropipette on each filter paper disc. The plates were sealed with paraflim immediately. Inoculated petri plates were incubated at 26+1°C in a BOD incubator The growth of the fungus was measured in mm at an interval of 24 hours (18).Percent inhibition of growth was calculated by using the following formula:

Where, I =lnhibition percentage, C = Colony radius in check (mm) T = colony radius in treatments (mm)

Results and Discussion

In present study, seeds of C. amada were extracted with six solvents with different polarity (petroleum ether, cyclohexane, ethyl acetate, chloroform, acetone and methanol) using Soxhlet apparatus. The yield of extracts mentioned in (Table 1). All the extracts were screened quantitatively in terms of their total phenols, flavonols and orthodihydroxy phenolic content with the help of their respective calibration curves (Figure 1). CAPE contained 91mg/100mg total phenols more than CAAC, CAEA, CAME, CACCL, CACH. CAME contained 26.31mg/100mg ortho dihydric phenolic contents more than CAPE, CAAC, CACL, CACH, CAEA. The flavonols content was observed more in CACH (75mg/100mg). The values are represented in catechol equivalent. The differences in the antioxidant activity of different extracts of C. amada may be possibly due to the different biochemical make up of the extracts in terms of phenols, flavonols and orthodihydroxy compounds and their concentration in the extracts.

The phytochemicals such as phenolics, flavanoid have been reported to reduce the oxidative peroxidation of lipids by possessing antioxidant activity [19-21]. The chemical composition and in vitro antioxidant potential of essential oil and rhizome extracts of Curcuma amada Roxb was reported in which essential oil was found to possess p myrcene over 40% as a major constituent (10). It has been reported by many workers all over the world that there exist a direct correlation among phenolic contents and antioxidant activity [22,23] (Figure 1).

For qualitative secondary metabolite profiling twelve phytochemicals viz. alkaloids, tannin, anthraquinone, glycosides, reducing sugar, saponins, flavonoids, terpenoids, caumarine, emodins, anthocyanin, and betacyanin were analyzed. The study revealed that alkaloids was present only in CAME. Tannin and saponins could not be detected in any extracts. Anthraquinone could be detected only present in CAEA. Glycosides and emodins were present in CAME. Betacyanin, caumarine and reducing sugars were present in CAEA. Flavonoids, terpenoids were present in all extract. Anthocyanin was present in CAPE, CACH, CAAC and CAME (Table 2).

The rhizome essential oil and all the extracts were found active against the tested fungi in dose dependent manner by inhibiting the fungal mycelia growth. All the extracts exhibited good to moderate activity against C. falcatum but the maximum inhibition dose recorded for CAEA (55.90%) at 1000ppm of dose level at 72 hrs. The other extracts inhibited 54.15-50.95% of mycelia growth in order of CACH (54.75%) >CAME (54.15%) >CAAC (53.38%) >CACL (51.05%) >CAPE (50.95%) respectively at the same dose level (Figure 2). Against S. solani all the extracts exhibited well to moderate activity but the maximum inhibition dose recorded for CAPE and CAEA (95.57%) at 1000ppm of dose level at 72 hrs. The other extracts inhibited 88.59 -73.89% of mycelia growth in order of CACL (88.59%) > CACH (82.86%) >CAME (73.95%) >CAAC (73.89%) respectively at 1000ppm of dose level at 72 hrs (Figure 2). CACH was found to be most effective against R. solani, it exhibited 100% growth at 1000ppm of dose level at 72 hrs.

The other extracts inhibited 79.78% - 46.70% of mycelia growth in order of CAAC (79.78%) > CACL (59.18%) > CAPE (55.66%) > CAME (53.40%) >CAEA (46.70%) respectively at 1000ppm of dose level at 72 hrs (Figure 2). Against S. rolfsii the extracts exhibited good antifungal activity but the maximum inhibition dose was recorded for CAME (93.25%) at 1000ppm of dose level at 96 hrs. The other extracts inhibited 68.00 - 49.34% of mycelia growth in order of CAPE (68.00%) > CAME (64.45%)>CACH & CACL (64.43%) >CAEA (49.34%) respectively at 1000ppm of dose level at 96 hrs As per the study the essential oil and all the extracts were found to active against the tested fungi (Rhizoctonia solani, Sclerotium rolfsii, Collectotricum falcatum and Sclerotenia solani) in dose dependent manner by inhibiting the fungal mycelia growth.

The essential oil of C. amada exhibited higher activity in comparison to extracts against all the fungi. The volatile oil from mango ginger rhizomes has antifungal in nature and it has been reported that myrcene and pinene possess antifungal activity against the wide range of fungi, viz. Curvularia palliscens, Aspergillus niger, A. terreus, Fusarium moniliforme and F. falcatum [24]. The major constituents of the essential oil have been reported by our group (10) also possess β-myrcene and β-pinene as the major constituent. Thus the present result of essential oil for possessing antifungal activity against tested pathogenic fungi was justified by the results reported earlier (Figure 2).

Conclusion

With the increased resistance towards synthetic drugs in phytopathogenic fungi, plant products may provide a better alternative to cure as well as prevent the infections caused by them. The present study revealed significant antifungal potential of Curcuma amada along with providing an easy, economic and less polluting way to extract out target bioactive molecules. However further investigations regarding the isolation of individual component from most active extracts may help in offering the natural alternative to treat infections caused by investigated fungus.

Acknowledgement

We are thankful to University Grants Commission (UGC), New Delhi. Thanks are due to the department of Plant pathology bio control lab, G B Pant University of Agriculture and Technology Pantnagar for antifungal activity determination.

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[marketfuturereports]

Dry Bath Incubator Market Size, Share and Competitive Landscape 2018-2025

12th September 2018 - Global Dry Bath Incubator Market is segmented into Types, Applications and Geographical regions. Dry bath incubators are referred to as laboratory devices that provide controlled environment for in-vitro diagnostic specimen and the other sample testing in tubes and micro tubes. Also known as dry block heaters, these devices are lengthily used in molecular biology and clinical and environmental laboratories. A dry bath is a type of laboratory equipment that is used to heat samples.

Access Dry Bath Incubator Market Report with TOC @ https://www.millioninsights.com/industry-reports/dry-bath-incubator-market

The capacity of these baths is measured in blocks. Depending on the size of the block, the user can place certain quantity of tubes into each block. The preponderance common sizes for blocks are 1, 2, and 4 block models. A dry block heater plays a vital role in incubation and activation of cultures, enzyme reactions, coagulation studies, and enzyme reactions, inactivation of Sera, restriction digests and Polymerase Chain Reaction, etc.

Dry Bath Incubator Market is segmented by Types into Analog Dry Bath Heather, Digital Dry Bath Heather, Heating Dry Block incubator, and Heated cooled Dry Bath Incubators. Digital dry baths incorporate a microchip into the model that allows the temperature of the bath to be controlled throughout the digital boundary though analog dry baths do not use microchip technology.

Heating Dry Block Incubator is the type of dry bath incubators that are designed for the applications that require heating temperature which is performing different tests. Fitted with microprocessor based circuit, these dry block heaters feature soft touch key panel, digital temperature display, buzzer alarm, and in-built over temperature protection. These versatile heating systems are available in single, double, and four blocks made of solid anodized aluminum. Each device offers outstanding temperature standardization ±0.5°C and works with ambient 5°C to 160°C temperature range

Users can interchange desired blocks as per their convenience. We have three standard models that accommodate dissimilar sample enclosures such as vials, micro plates, PCR strips, and a wide assortment of tubes. Heated Cooled Dry Bath Incubators is the dry bath incubators that combine heating and cooling in one device. These systems are suitable for molecular biology applications that require close to freezing environment.

Designed for long term steady operation, these devices could be operated in heating or cooling mode as required. In accumulation, there are dissimilar models of dry bath incubators; which also features digital display touch key panel and transposable blocks. This device is ideal for maintain 14°C for storing oocyte.

The major manufacturers covered in this report

• Thomas Scientific

• Major Science

• Boekel Scientific

• LW Scientific

• M.R.C

• Torrey Pines Scientific, Inc.

On the basis of product, this report displays the production, revenue, price, market share and growth rate of each type, primarily split into

• Single heater block

• Double heater blocks

• Four heater blocks

On the basis of the end users/applications, this report focuses on the status and outlook for major applications/end users, consumption (sales), market share and growth rate for each application, including

• Clinical

• Biology

Request a Sample Copy of Dry Bath Incubator Market Report @ https://www.millioninsights.com/industry-reports/dry-bath-incubator-market/request-sample

Geographically, this report is segmented into several key Regions, with production, consumption, revenue (million USD), market share and growth rate of Dry Bath Incubator in these regions, from 2013 to 2025 (forecast), covering

• North America

• Europe

• China

• Japan

• Southeast Asia

• India

[hlscientificind]

Laboratory Accessories Manufacturer in India - HLscientificind

HL scientific Industries is one of the leading Brand of Laboratory Accessories Manufacturer in Haryana India. It produces different type of laboratory accessories varities like Anemometer Cup Type, Dry Bath Incubator, Test Tubes Stand etc. Along with this it manufacture's  Laboratory Thermometer, Laboratory Instruments etc.

https://hlscientificind.com/product-category/laboratory-accessories-manufacturer-in-india/