#Co2 Incubator Manufacturers

[microsilindia]

What is the Purpose of a Co2 Incubator?

CO2 incubators provide optimal conditions for cell culture, maintaining precise levels of temperature, humidity, and carbon dioxide. These controlled environments support cell growth and viability, crucial for various research and clinical applications. With advanced features like direct heat and infrared sensor technologies, they ensure uniform and stable conditions, fostering reproducible results. Microsil India offers high-quality CO2 incubators designed to provide the ideal environment for cell culture growth. Trust Microsil India for reliable CO2 incubators that ensure optimal conditions for your research and experimentation needs.

[kimberlabtron]

Labtron Air Jacketed CO2 Incubator LAJI-C10 offers outstanding temperature stability. provides a heating method that is air-jacketed.Temperature Range: RT+5 °C to 50 °C. Temperature Gradient: ≤ ± 0.5 °C. Electrical Requirement: AC220 V, 50 Hz Power Consumption: 400 W. ISO Class 5 chamber with air renewal system. humidity sensor with water top-up alarm Meets sterilization of 99.99% with 80°C heat on cells. CO2 pressure-relief valve with 2-stage control and stable pressure.

[yathermscientific]

CO2 Incubator Troubleshooting: Common Problems and Solutions

Is your CO2 incubator giving you trouble? Check out this guide for common problems and solutions to keep your experiments running smoothly.

A CO2 incubator is a crucial piece of equipment for many scientific experiments, but it can be frustrating when it's not working properly. This guide will help you troubleshoot common issues with your CO2 incubator and provide solutions to keep your experiments running smoothly.

Temperature fluctuations

One common problem with CO2 incubators is temperature fluctuations. This can be caused by a variety of factors, including a malfunctioning thermostat, a faulty heating element, or poor insulation. To troubleshoot this issue, first check the thermostat and make sure it is set to the correct temperature. If the thermostat is working properly, check the heating element and make sure it is functioning correctly. If neither of these solutions work, it may be necessary to replace the insulation or contact a professional for further assistance.

CO2 level fluctuations

Another common problem with CO2 incubators is fluctuations in CO2 levels. This can be caused by a variety of factors, including a malfunctioning CO2 sensor, a clogged gas inlet, or a leak in the system. To troubleshoot this issue, first check the CO2 sensor and make sure it is functioning properly. If the sensor is working correctly, check the gas inlet and make sure it is not clogged. If neither of these solutions work, it may be necessary to check for leaks in the system or contact a professional for further assistance.

Contamination

Contamination is a common problem in CO2 incubators and can be caused by a variety of factors, including improper cleaning, contaminated media or reagents, or a malfunctioning HEPA filter. To prevent contamination, it is important to regularly clean and disinfect the incubator and all equipment used inside. Additionally, make sure to use sterile media and reagents and regularly replace the HEPA filter. If contamination does occur, it may be necessary to discard any affected samples and thoroughly clean the incubator before starting again.

Read more: Co2 Incubator-About, Benefits & Where to Buy

Water accumulation

Water accumulation is a common problem in CO2 incubators and can be caused by a variety of factors, including condensation from high humidity levels or a malfunctioning water pan or drain. To prevent water accumulation, make sure to regularly check and empty the water pan and ensure that the drain is functioning properly. Additionally, reducing the humidity levels in the incubator can help prevent condensation. If water accumulation does occur, it is important to thoroughly clean and disinfect the affected areas to prevent contamination.

Door seal issues

One common problem with CO2 incubators is door seal issues. If the door seal is not functioning properly, it can lead to temperature and humidity fluctuations, which can negatively impact your experiments. To check for door seal issues, inspect the seal for any cracks or gaps. If you notice any issues, try cleaning the seal with a mild detergent and warm water. If the problem persists, you may need to replace the seal. It is important to address door seal issues promptly to ensure the integrity of your experiments.

[bhushans]

The Future of Autism Care: Global Cell Culture Incubator Market

The cell culture incubator market is on track for substantial expansion, with a projected value of USD 2,315.5 million in 2023. Over the next decade, the market is expected to double in value, reaching an impressive USD 4,305.1 million by 2033. This growth will be driven by a Compound Annual Growth Rate (CAGR) of 6.4% during the forecast period, building upon a solid foundation of 5.6% CAGR observed from 2018 to 2022.

Cell culture incubators play a critical role in scientific research, pharmaceutical development, and biotechnology applications, providing controlled environments for cell growth, maintenance, and experimentation. As the demand for cell-based research and biopharmaceutical production continues to rise, the market for cell culture incubators is experiencing robust growth.

Request Your Detailed Report Sample With Your Work Email: https://www.futuremarketinsights.com/reports/sample/rep-gb-4392 

Bacteria and cell cultures are kept growing inside a chamber at carefully regulated temperature, humidity, pH, and CO2 concentration by a device called a cell culture incubator. The demand for more vaccines based on cell culture and its subsequent approvals are fueling the market for cell culture incubators. The rising prevalence of chronic illnesses and the growing need for monoclonal antibodies are predicted to drive growth in the target market.

A specialized scientific tool for the meticulously regulated development and preservation of cells is a tissue culture incubator. To promote the ideal conditions for cell formation and proliferation, it offers stable settings with regulated temperature, humidity, and gas concentrations.

Market Competition:

The key players of this market include Thermo Fisher Scientific Inc, Memmert GmbH + Co.KG, Binder GmbH, Panasonic, BioIVT, Sheldon Manufacturing, Inc.

Thermo Fisher Scientific Inc. announced in June 2021 that the company’s Thermo Scientific Cytomat 24 automated incubators and storage systems are combined with the latest technology to aid increased throughput and sample protection. The new Cytomat 24 automated incubators and storage systems from Thermo Fisher Scientific, which will be available in May 2021, provide a large capacity, quick access, and a wide temperature range while minimizing contamination difficulties in high-throughput applications. The new instruments use temperature consistency and stability to assure reproducibility for cell culture applications, while also providing speedy delivery of microtiter plates via an enhanced plate shuttle system, according to a press release issued by Thermo Fisher on May 14, 2021. The system also incorporates an LED touch screen that is installed on the door for easy access and viewing.

BioIVT released its GMP Grade Human AB Serum tailored for gene therapy manufacturing in January 2022. BioIVT’s GMP-grade human AB serum is manufactured with the same optimized processes and improved regulatory control of source material collection, manufacturing, and processing as its research-use only material. However, it also includes extra viral tests and documentation for hepatitis A (plasma-derived only), hepatitis B core antibody, hepatitis E, and parvovirus B19 (plasma-derived only).

Key Segments Profiled In The Cell Culture Incubator Industry Survey:

By Type:

Air-jacketed

Water-jacketed

Direct Heat

By Sensor Technology:

Infra-red

Thermal Conductivity

By Application:

For Pharmaceutical Applications

For Biomedical and Clinical Labs

For Government Research

For IVF Processes

For Other Applications

By Region:

North America

Latin America

Europe

Asia Pacific

Middle East & Africa

[tmmedia17]

Check the Best Quality Blood Culture Specimen Collection (TMC 074) at TM Media

If you are looking for blood culture specimen collection (TMC 074) manufactured by TM Media automates the process of detecting microbial growth in blood samples. It typically consists of a sterile chamber where blood culture bottles are incubated and monitored for bacterial or fungal growth. The instrument continuously scans the bottles for signs of growth, such as changes in CO2 levels or turbidity. Once growth is detected, the instrument alerts laboratory staff, facilitating prompt identification of bloodstream infections. This automated system improves efficiency, reduces manual labor, and enhances the accuracy of blood culture analysis, leading to faster diagnosis and appropriate treatment of infections.

Visit the Website - https://www.tmmedia.in/

[labequipmentnl]

Exploring the Evolution of Laboratory Ovens: Precision, Efficiency, and Innovation

Laboratory ovens are indispensable tools in scientific research, pharmaceuticals, and industrial applications, providing controlled heating environments for various processes such as drying, curing, sterilization, and testing. Over the years, these ovens have undergone significant advancements, driven by the need for precision, efficiency, and innovation.

Precision is crucial in laboratory operations, and modern laboratorium oven are equipped with advanced temperature control systems that ensure precise and uniform heating. Microprocessor-based controllers, thermocouple sensors, and PID algorithms enable users to set and maintain precise temperature levels with minimal fluctuations, ensuring consistent and reliable results.

Efficiency is another key consideration in laboratory operations, and manufacturers have made significant strides in improving the energy efficiency of laboratory ovens. Insulated chambers, sealed doors, and energy-efficient heating elements minimize heat loss and reduce energy consumption, making modern ovens more environmentally friendly and cost-effective to operate.

Innovation drives the evolution of laboratory ovens, pushing the boundaries of what is possible in terms of functionality and performance. Recent innovations include the integration of touchscreen interfaces, programmable controls, and remote monitoring capabilities, allowing users to easily program and monitor oven operations from anywhere. Additionally, the introduction of multi-functional ovens that combine heating, drying, and sterilization capabilities in a single unit provides greater versatility and efficiency in laboratory workflows.

Safety is a paramount concern in laboratory settings, and manufacturers have implemented various safety features to protect users and samples. These features may include overtemperature protection, door interlocks, and alarms to alert users of any deviations from set parameters, ensuring safe operation and preventing potential hazards.

Conclusion :

laboratory ovens have evolved significantly to meet the demands of modern scientific research, pharmaceuticals, and industrial applications. The latest advancements in precision, efficiency, innovation, and safety ensure that laboratory ovens remain essential tools for a wide range of heating and drying processes. As technology continues to advance, we can expect further enhancements that will drive the evolution of laboratory ovens and improve their functionality, performance, and usability.

For More Info :- 

Co2 incubator

Incubator

[labexpo254]

Water Jacketed Co2 Incubator - Labexpo USA

Microprocessor PID controlled CO2 Incubator ensures optimum environment for cell and tissue culture growth. It is made up of corrosion resistant stainless steel and provides protection against over temperature and current leakage. These CO2 Incubator delivers a higher level of performance for a dependable and reliable in-vitro growth environment through water jacket that provides true temperature uniformity and CO2 gas control. This incubator features unsurpassed temperature stability and superior parameter recovery.

[yathermscientific1]

Understanding the Importance of CO2 Incubator Shaker

Get the optimum CO2 cultivation environment for your cannabis grow with our CO2 incubator shaker!

[kdmglobal]

Ambient Air Quality Monitoring System Manufacturers in India

The founders of KDM Global have more than 40 years of combined experience in the design, manufacture, marketing, and service of environmental test chambers. Their goal is to provide high-quality products at competitive prices while utilising creative cost-cutting measures.

KDM Global is a renowned manufacturer, exporter, and supplier of laboratory and scientific equipment of the highest calibre. We offer a variety of products, such as laboratory equipment, scientific equipment, low temperature equipment, clean room equipment, a laboratory autoclave that is vertical, a stability chamber, a humidity oven, an orbital shaker incubator, a vertical dehumidifier, a rotary shaker, a CO2 incubator, a fume chamber, a deep freezer, a blood bank refrigerator, and a UV inspection cabinet,Vertical Reverse Flow, Clean Air Pressure Modules, and Biological Safety Cabinets Bio-Hood. Powder Dispensing Booth. We have been able to keep up with the continuously changing requirements of numerous industries by drawing on our years of experience. Recent technological advancements have compelled us to create, customise, and manufacture equipment that meets the unique needs of various laboratories and governmental organisations. Due to our dedication to quality and adherence to international quality standards, we have been able to carve out a space for ourselves in this business internationally.

Our items are made from excellent materials that have been examined for effectiveness and sturdiness. Our highly skilled and knowledgeable employees can create the accessories to the clients' demands. Our product lines are made from premium materials that have been evaluated for their effectiveness and quality. They are created in accordance with the demands of the customers. We offer both carton and wooden packaging according on the needs of the products.

OUR PURPOSE

o In order to give creative solutions for product development and quality assurance, it is our objective to design, build, and supply dependable environmental test chambers in conjunction with our clients.

· As a well-known supplier of top-notch solutions in the reliability testing industry, to offer clients who are concerned about quality with solutions that are accurate, unbiased, and give them a competitive edge over their rivals.

o We are dedicated to offering our clients across the globe unrivalled customer service. We promise great customer service in terms of product delivery, product information, and online/offline support, whether it is a domestic or international firm.

o Motivate and educate our staff and channel partners about the value of our testing processes in the creation and dependability of our clients' products.

We monitor air quality because

Designing and producing the best air quality monitoring technology is at the heart of KDM Global's aim to strike a balance between development and preservation, which supports our dedication to fostering surroundings that are conducive to possibility.

The most fundamental goal of air quality monitoring is to protect lives and keep you safe from dangerous gases, greenhouse and trace gases, particles, dust, and smoke that may have a negative impact on your health, happiness, quality of life, and longevity.

For more  details contact us:

Email : saleskdmglobal@gmail.com

Website:https://kdmglobal.business.site , https://sites.google.com/view/kdmglobal/home

Contact :8218470498

[mohdasik7]

ALLPHA LAB INSTRUMENTS

Allpha is a India company that manufactures an extensive range of Laboratory Equipment and Analytical instruments.We aim to enhance research and development across various fields of Life Sciences and allow access to the best and cost-effective equipment. our products are tested and approved by experts from R&D, Quality Assurance, and Quality Control and are at par with European Standards including ISO and CE certifications

Having built a strong network of professionals over the years, we now sell instruments and consumables. Their reputed clients and manufacturers appreciate the time and money they spend on them, which is why they stay up-to-date with the latest technology. Over the past years, Allpha has worked with industry leaders to design solutions for production and research needs across the entire constant temperature / environment spectrum.The company has a wide range of laboratory equipment used in research, light industry, medical and healthcare, and education Laboratory equipment from our company is manufactured in our manufacturing facilities located throughout India and worldwide. Customized equipment can also be manufactured to fit your specific requirements.All of their raw materials are high-quality ones sourced from reliable sources, which allows them to be qualitative and long-lasting. Customized and developed based on customer specifications, these products are made to fit their needs. Every product is carefully scrutinized to guarantee that the best products can be supplied to the customers.

Allpha is founded By a qualified engineer with experience in Scientific Instrumentation industry in India from 2018 lab instruments in chennai. Allpha Incorporated in 2018, an ISO 9001:2015 certified manufacturer of high quality and innovative laboratory and scientific equipment for the global market. We have expertise in innovative product manufacturing that adds value to the customer’s portfolio.High-quality, durable, efficient equipment is widely demanded and appreciated for its high performance, reliability, durability, and efficiency. They keep themselves informed about new developments related to their products’ design and construction through continuous research and development.

The range of products include

-40°C Freezer

-86°C ULT Freezer

CO2 Incubator

CO2 Incubator Air Jacketed

CO2 Incubator Water Jacketed

Shaking Incubator

Water Bath

Cloud And Pour Point Apparatus

Lyophilizer / Freeze Dryer

Laminar Air Flow

Horizontal Laminar Air Flow

Vertical Laminar Air Flow

Fume Hood

PCR Cabinet

Biological Safety Cabinet

Class I Biosafety Cabinet

Class II Biosafety Cabinet

Class III Biosafety Cabinet

Lab Furntures

Allpha markets a complete line of products under Allpha brand which complement our OEM manufacturing capacities. Allpha’s reputation has been built on innovation, high quality and long term partnering. Allpha focuses to provide economical equipments to Research institutions, Univertities & colleges & manufacturers in industries such as electronics, Pharmaceutical Research, Cement, Steel etc. allpha has established its manufacturing facility at Chennai. The infrastructure is facilitated with sophisticated fabrication, Electrical & Refrigeration department with expert engineering team, well equipped and trained Quality control department.

[petnews2day]

Increased autophagy leads to decreased apoptosis during β-thalassaemic mouse and patient erythropoiesis

New Post has been published on https://petnews2day.com/pet-news/small-pet-news/increased-autophagy-leads-to-decreased-apoptosis-during-%ce%b2-thalassaemic-mouse-and-patient-erythropoiesis/

Increased autophagy leads to decreased apoptosis during β-thalassaemic mouse and patient erythropoiesis

Animals

This study was approved by the Mahidol University Animal Care and Use Committee (approval number MU-ACUC 2009/001). All methods were performed in accordance with the relevant guidelines and regulations. Wild type mice and βIVS2-654-thalassaemia in C57Bl/6 background mice20 were employed in the present study. Murine bone marrow cells were harvested from femurs and tibias as described in a previous study21. All methods are reported in accordance with Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines and American Veterinary Medical Association (AVMA) Guidelines for the Euthanasia of Animals (2020).

Flow cytometric analysis of murine erythroid apoptosis

Erythroid subpopulations were defined as described in previous studies18,26. Whole bone marrow samples were stained with fluorochrome conjugated monoclonal antibodies specific to CD71 (transferrin receptor, BD Biosciences, San Jose, CA) and TER119 (red cell marker, BD Biosciences). Then, samples were combined with a third-color fluorescent channel staining such as fluorochrome conjugated annexin V (phosphatidylserine marker, BD Biosciences) or 100 nM 3,3′-dihexyloxacarbocyanine iodide (DiOC6(3)) (Sigma-Aldrich, St. Louis, MO) to determine mitochondrial transmembrane potential. For determination of activated caspase 8 and caspase 9 in living cells, bone marrow samples (2 × 106 cells) were incubated with either fluorescein isothiocyanate (FITC)-conjugated IETD-FMK (for caspase 8, Calbiochem, San Diego, CA) or sulforhodamine (Red)-conjugated LEHD-FMK (for caspase 9, Calbiochem) following the manufacturer’s recommendation. The cocktail of monoclonal antibodies that was used in this study are shown in Supplementary Table 1. Data of 100,000 events was acquired and analysed using a BD FACSCalibur flow cytometer and CellQuest Pro™ software (BD Biosciences). K562 erythroleukemic cells treated with 193.8 mM hydrogen peroxide (H2O2) for 15 min at 37 °C, 5% CO2 were used as a control.

Murine bone marrow erythroblast isolation

CD45− erythroblasts and CD45–CD71+ erythroblasts from bone marrow samples were isolated using a MACS cell separation system (Miltenyi Biotec, Bergen Gladbach, Germany) as the manufacturer’s instructions. The purity of murine CD45–CD71+ bone marrow erythroblasts was 73–91% as measured by flow cytometry.

Transmission electron microscopic analysis of murine erythroid autophagy

Bone marrow erythroblasts were harvested and analysed for morphology and LC3 expression using a transmission electron microscope41. CD45– bone marrow erythroid cells (1 × 107 cells) were fixed in 2.5% glutaraldehyde solution for 4 h at 4 °C, then, washed with Sorenson’s phosphate buffer and stained with 1% Caulfield’s osmium tetroxide supplemented with sucrose for 1 h. Samples were dehydrated with different concentrations of ethanol and finally propylene oxide, then, embedded in Embed 812 resin BEEM capsules (Electron Microscopy Science, Hatfield, PA) at 65–80 °C for overnight. Thin section (60–90 nm) was collected and mounted on copper grids, stained with uranyl acetate, and counterstained with lead citrate. Grids were observed and images were obtained using a transmission electron microscope (Hitachi H-7100, Hitachi High-Tech Science Corporation, Tokyo, Japan) operated at 100 kV. Images were captured at 4000 × magnification for 10 fields per sample by sequential field collection. Autophagic vacuoles/autolysosomes was defined as membrane-bound vacuole containing electron-dense cytoplasmic material and/or organelles at various stages of degradation. The electron-lucent cleft between the two limiting membranes was used to aid identification. The area of autophagic vacuoles and the total cytoplasmic area of erythroblasts were measured using the Scion Image Beta 4.02 analysis software (Scion Corporation, Chicago, IL). A total of 50 cells per sample were analysed.

For LC3 expression determination by immunogold staining41, a thin section of CD45– bone marrow erythroid cells from β-thalassaemic mice processed for transmission electron microscopy were collected and mounted on nickel grids. Grids were blocked with 0.5% BSA-C (Aurion, Wageningen, Netherlands), and then stained with a rabbit anti-LC3 polyclonal antibody (Aurion) as a primary antibody and gold-conjugated F(ab)2 fragments of goat anti-rabbit IgG (Aurion) as a secondary antibody, and counterstained with uranyl acetate. Control samples used rabbit serum (Dako, Glostrup, Denmark) in place of the primary antibody were used as a negative control. Images were captured using a transmission electron microscope (Hitachi H-7100) operated at 100 kV.

Confocal microscopic analysis of murine erythroid autophagy

CD45– bone marrow erythroid cells (3 × 105 cells) were fixed with 4% paraformaldehyde and blocked with 1% BSA-C solution (Aurion) for 1 h. Subsequently, samples were permeabilized with 0.3% Triton X-100 in phosphate buffered saline and incubated with a rabbit anti-LC3 polyclonal antibody (Novus Biologicals, Littleton, CO), then, subsequently a Cy5-conjugated goat anti-rabbit IgG (H+L) F(ab’)2 fragments (Invitrogen, Carlsbad, CA). After incubation and wash, samples were stained with FITC conjugated rat anti-LAMP-1 monoclonal antibody (Biolegend, San Diego, CA). Fluorescent signal was observed using an Olympus FluoView 1000 confocal microscope (Olympus, Tokyo, Japan). Illustration was captured using a 60 × objective lens for 10 field per sample by sequential field collection and discard 10 fields. A total of 50 cells per sample were analysed. Image analysis and calculation of Pearson’s correlation coefficients and confidence intervals were undertaken as previously described17,42. Autophagosomes with punctate LC3 expression in erythroblasts were count and calculated as the percentage of autophagic cells in total erythroid cells (Supplementary Fig. S4).

Inhibition autophagic flux of murine erythroblasts

CD45–CD71+ bone marrow erythroblasts were cultured in Iscove’s Modified Dulbecco’s medium (IMDM) supplemented with 20% fetal bovine serum (FBS) with or without 100 μM chloroquine at 37 °C, 5% CO2. Chloroquine was dissolved in culture medium and filtered through a 0.2 μM filter before use. The cells were collected at 3 h. after treatment for analysis of LC3-I and LC3-II by western blot43 and at 24 h. for determination of PS expose by flow cytometry.

Western blot analysis of erythroid autophagy

CD45–CD71+ bone marrow erythroid cells were lysed and sonicated in lysis buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 0.1% SDS and 1% Triton X-100) supplemented with protease inhibitor cocktail (Sigma-Aldrich). A total of 50 μg protein of the lysates was separated through 15% denaturing polyacrylamide gels and transferred to polyvinylidene difluoride membrane (Bio-Rad Laboratories). Primary antibodies used were a rabbit anti-caspase 3 polyclonal antibody (Cell Signaling Technology, Danvers, MA), a rabbit anti-LC3 polyclonal antibody (Abcam, Cambridge, UK) and a rabbit anti-β-actin monoclonal antibody (Sigma-Aldrich). Secondary antibody was used was a horseradish peroxidase conjugated goat anti-rabbit IgG (Sigma-Aldrich). The densitometric analysis was performed using ImageJ software (The National Institutes of Health, MD).

Human bone marrow collection

This study was performed in accordance with the Helsinki declaration and was approved by the Mahidol University Institutional Review Board (approval number MU-CIRB 2014/031.1703). Written informed consent was obtained from all individual participants in this study. Patients under treatment with hydroxyurea, aspirin, antibiotics, anti-depressants, beta-blockers and anti-platelets were excluded, and no participant had been hospitalised or transfused within 4 weeks of sample collection. Human bone marrow samples were collected from 6 β-thalassaemia/HbE patients and 3 control subjects into citrate phosphate dextrose adenine solution anticoagulant by using the bone marrow aspiration technique. Samples were processed within 1 h after aspiration. All methods were performed in accordance with the relevant guidelines and regulations. Haematological parameters are shown in Supplementary Table 2.

Human bone marrow erythroblast isolation

CD45– erythroblasts from bone marrow samples were isolated using a MACS cell separation system (Miltenyi Biotec) as the manufacturer’s instructions. The purity of human CD45– bone marrow erythroblasts was 45–70% as measured by flow cytometry.

Flow cytometric analysis of human erythroid apoptosis

Erythroid subpopulations were defined as described in previous studies17,27. Whole bone marrow samples were stained with fluorochrome conjugated monoclonal antibodies specific to CD45 (leukocyte marker, BD Biosciences), CD71 (BD Biosciences) and glycophorin A (GPA, red cell marker, BD Biosciences). Then, samples were combined with a fourth-color fluorescent channel staining such as fluorochrome conjugated annexin V (BD Biosciences) or 50 nM tetramethylrhodamine ethyl ester perchlorate (TMRE) (Sigma-Aldrich) for mitochondrial transmembrane potential. Whole bone marrow samples were incubated with 100 μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 30 min at 37 °C, 5% CO2 for direct disruption of mitochondrial transmembrane potential and this was used as a control.

For intracellular staining to determine active caspase 3, whole bone marrow samples were incubated with cold-cytoFix/Perm (BD Biosciences) for 10 min at 4 °C and then were stained with fluorochrome conjugated monoclonal antibodies specific to CD45, CD71, GPA and either activated caspase 3 (BD Biosciences) or isotype control for 30 min at 4 °C. Data of 100,000 events was acquired and analysed using a BD FACSCalibur flow cytometer and CellQuest Pro™ software. Bone marrow sample treated with 193.8 mM H2O2 for 15 min at 37 °C, 5% CO2 was used as positive control. The cocktail of monoclonal antibodies that was used in this study are shown in Supplementary Table 1.

Human erythroid cell autophagic analysis

CD45– human bone marrow erythroblasts were isolated and analysed co-localization of LC3/LAMP-1 using confocal microscope17,42. CD45– human bone marrow erythroblasts (3 × 105t5 cells) were fixed and stained with a rabbit anti-LC3 polyclonal antibody (Novus Biologicals), then, subsequently a Cy5-conjugated goat anti-rabbit IgG (H+L) F(ab’)2 fragments (Invitrogen) as secondary antibody, after incubation and wash, samples were incubated with a FITC conjugated rat anti-LAMP-1 monoclonal antibody (Biolegend). After incubation and wash, samples were stained with 4′,6-Diamidino-2-phenylindole, dihydrochloride (DAPI, Molecular Probes, Eugene, OR). Images were captured using a 60 × objective lens for ≥ 10 field per sample. A total of 50 erythroid cells per sample were analysed. Fluorescent signal was observed and analysed as described in the section of “Confocal microscopic analysis of murine erythroid autophagy” (Supplementary Figs. S8 and S9).

Statistical analysis

Data were analyzed using SPSS Version 18.0 (SPSS Inc., Chicago, IL). Comparisons between parameters which analysed using flow cytometry and cell counting were evaluated by Mann–Whitney U test. Comparisons between parameters which image analysis including autophagic vacuole per cytoplasmic area ratio and densitometric analysis of protein expression using Western blot were evaluated by independent Student’s t-tests. The threshold for statistical significance for all comparisons was P < 0.05.

[microsilindia]

Incubator Manufacturer in India

With an emphasis on cost-effective yet efficient products, Indian incubator companies are focused on providing high-quality and reliable equipment for clinical and research purposes. These manufacturers offer a range of different types of incubators including microbiological, neonatal, cell culture and CO2 incubators. Microsil India ( Linco Scientific ) is one of the leading incubator manufacturer in India. They are designed to provide a safe, controlled environment for the growth and development of cells, tissues, and organisms. Contact for more!

Visit: https://www.microsilindia.com Contact: +91-9112862222 Mail: lincosci@gmail.com

[yathermscientific]

Yatherm manufactures customized Orbital shaker, VDRL Shaker, rotary flask shaker, co2 incubator shaker, Wet Sieve shaker Yoder type, Sedimentation shaker, incubator shaker & Tabletop electromagnetic laboratory sieve shaker machine for Research applications.

https://www.youtube.com/watch?v=ntA9EN1tdmo

[ahmed25646]

The recovery of CO2, incubator of innovations

The recovery of CO2, incubator of innovations

The storage and sequestration of CO2 make it possible to reduce its atmospheric concentration. More and more technologies today offer CO2 a second life as a raw material. CO2 is gaining ground. Yesterday considered as ultimate waste, it is now the subject of particular attention from manufacturers, in France and around the world. The latter are developing technologies to capture it, valorize it,…

[cleatechlaboratory]

The role and uses of nitrogen gas generators

The risk of nitrogen leaks is higher, which can be deadly if left unnoticed. Storing larger amounts of pressurized, extremely flammable nitrogen in labs also increases the risk of fire & explosions.  Our Nitrogen Gas Generator lessens these risks. It is compact and easy to operate, and they allow lab personnel to produce nitrogen at the bit of a button. Purity levels are also considered, with the most advanced instruments generating nitrogen of 99% purity.

Our consistent & cost-effective source of nitrogen      

Consistency is one of the chief benefits of nitrogen gas generators. Unlike pressurized gas tubes which can take on impurities as they age, Nitrogen Gas generators deliver a constant supply of pure, and lab-standard gas. This stops workflow disruptions and progresses lab productivity across the board. Investing in a Nitrogen Gas Generator can also slash the operational costs related to running labs. Instead of purchasing the gas and paying for cylinder distributions and deposits, Nitrogen Gas Generator delivers medical-grade nitrogen on demand every time. Our leading Nitrogen Gas Generator manufacturers many instruments at a very reasonable price.

Along with nitrogen, hydrogen is also one of the most widely used lab gases. The Nitrogen Gas Generator, as well as other instruments, are designed to remove filths such as water vapours, methane & CO2 from ambient air.

Low reactivity with additional compounds & the ability to control ambient oxygen levels makes nitrogen the gas of choice for countless applications. From carrying out Liquid Chromatography and Mass Spectrometry analysis to operating Evaporative Light Scatter Detectors and maintaining ecological conditions in Vitro Fertilisation incubators, the ability to produce nitrogen on demand is essential in many labs.

Want to purchase the best quality and efficient Nitrogen Gas Generator at a reasonable price?

If you want to purchase the best quality Cleatech Nitrogen Gas Generator at a reasonable price for your lab, you can contact us at any time.

Original Sources: https://laboratoryquipment.blogspot.com/2022/09/the-role-and-uses-of-nitrogen-gas.html

[gauri1127]

Here you can find broad list of CO2 Incubator manufacturers, dealers and suppliers from India, as well as all Healthcare related products and services on Hospital Product Directory.