#Amplifx pcr

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Amplifx pcr

#AMPLIFX PCR SOFTWARE#

#AMPLIFX PCR CODE#

To validate this assay, serum samples were collected from patients infected, either acutely or chronically, with HBV or HCV and maintained at -20 Â☌ until use. Popular search Validation Of The Assay With Serum Samples All data is backed up multiple times a day and encrypted using SSL certificates.

#AMPLIFX PCR CODE#

Our internal code of conduct adds additional privacy protection. We use procedural, physical, and electronic security methods designed to prevent unauthorized people from getting access to this information. We’ve put industry-leading security standards in place to help protect against the loss, misuse, or alteration of the information under our control. You May Like: Hepatitis C And Liver Failure We Implement Proven Measures To Keep Your Data SafeĪt HealthMatters, we’re committed to maintaining the security and confidentiality of your personal information. The extracted DNA and RNA were stored at -80 Â☌ until use. The concentration and quality of the extracted DNA and RNA were assessed by Nanovue spectrophotometry and by amplification of a fragment of the gene coding for β-actin. Nucleic acids were extracted from 200 µL of serum using a QIAamp MinElute Virus Spin kit according to the manufacturer’s suggested protocol.

#AMPLIFX PCR SOFTWARE#

All of the primers were selected using the primer premier 5.0 software and were synthesized, purified and labeled by Takara Ltd. The fluorophore primers were quenched by partly complementary oligonucleotides of a single-base mismatched labeled with a quencher at 3′-end. The fluorophore primers and the reverse primers were used to amplify a 94-bp fragment in the highly conservative 5′ non-coding region of the HCV RNA. The baseline and threshold values were automatically adjusted for each test. Results were analyzed using 7500 Fast Software v. All reactions were performed in duplicate using universal conditions: 50 Â☌ for 2 min, 95 Â☌ for 10 min, 45 cycles of 95 Â☌ for 15 s, and 60 Â☌ for 1 min. The reactions were performed in a 7500 Real-Time PCR System using the TaqMan detection system with 12.5 µL of TaqMan Universal Master Buffer, predetermined concentrations of the primer-probe sets cited above, and 50 – 100ng of DNA or cDNA, for a total final volume of 25 µL per reaction. Blood Test : HCV RNA By Real Time PCR Quantitativeįor validation of the assay, the international panel cited above was used in qPCR reactions to construct standard curves of HBV DNA or HCV RNA in the following concentrations: HBV 7 or HCV 7, HCV 6 or HBV 6, HBV 5 or HCV 5, HBV 4 or HCV 4, HBV 3 or HCV 3, and HBV 2 or HCV 2.