#ATCC

[wackysach]

guess it's time to make a post about this because i realize i never actually did... so yeah ! uhm, ATCC was a... thing, and it's most likely the thing that many of my followers now remember me for (and if you're out of loop with it, ATCC or @ask-the-creative-crew was a DHMIS centered ask blog i made back in the year 2022), and it's been a hot minute since i've touched on it.

originally, i stopped posting because i was still in the school at the time and the blog was something i did on the side for fun. it only ever came to fruition because i wanted another place to show off my designs and headcanons other than instagram (RIP to the gram though). my intent for it then was just to be a silly thing that had no "heavy" storyline to it other than a bunch of little guys all hanging out together.

some time later, the TV show released and changed my perspective on a lot of the characters as well as the story (which i had begun to build whenever i was taking long breaks). fast forward 2 years and some emotional growth and artistic improvement - much of the story and character designs/personalities/relationships have evolved to fit the new narrative i've built. a lot of change has happened and will most likely keep happening as long as B&J keep producing the show and giving us new content to work with.

i mention all of this because a lot of what's on the blog (or what little of it was even there to begin with) doesn't exactly align with what i have now. i don't want to say it's dead forever and ever, but rather in an indefinite limbo while i try to figure out how i want to go about sharing the new story. i tend to be a little cryptic when sharing details about what it's like now because things are still susceptible to change, and also because i don't want to reveal the whole thing yet, but i really am more than happy to answer any questions you guys have about it (just don't always expect me to provide art if i answer. i'll give you rabies if you beg. rawr).

it does kind of hit a soft spot in my heart to know there are still people interested in my goofy little interpretations. thank you to everyone who's been supporting me after all these years, and for reading my ridiculously long explanation. have a cookie. 🍪

[jackobbit]

Alrighty, here we go, one last time!

We’ve got some feesh, and this pumpkin fella I’ve been rotating in my brain all week. (I do apologize for the low quality on that last one especially, I had to rush at the end bc I had some other stuff to do)

With this, the week of au’s Magma event is over! It was super fun to to draw everyday with other folks! Seeing everyone else’s art was a real treat.

It also provided a nice challenge! It really did help me learn a lot, but, I think I’m ready to use my usual programs again lmao

[ID: A Magma drawing of a Mer Sun and Mer Moon underwater, they are both placed against a blue-green background with both Sun and Moon being drawn slightly outside of the colored box. Mer Sun is yellow and orange with lots of dark brown spots, he waves his arms up and down quickly as he looks up and to the right at Moon who floats overhead. He is smiling brightly. Moon looks down at Sun with a smile, one hand is stretched out towards Sun while his other is kept in a fist near his chest. Moon is colored a mix of greys and blues with brighter electric blue spots. /End ID]

[ID: A Magma drawing of a Halloween version of Sun sitting on a hay bale within a corn field. He has a pumpkin for a head, and wooden sun-like rays. He wears a witch’s hat, a burlap shirt, black pants, gloves, a belt and boots. He smiles, looking off into the distance. Next to him is a small pumpkin on the ground, carved to resemble a grinning crescent moon. The background consists of an orange sky, clouds, a full moon and two bats flying in the sky. /End ID]

[literarynecromancy]

All Things Concerning Chaos: Main Cast

(An "A" beside the name denotes an Antagonist)

Aelyn

Born into a chaos worshiping cult that lives below Icarus. Was raised and shaped to be the next leader, and decided to leave gender behind in the process. Was sent out to track the group to find their deity who was recently released.

Darien (A)

From the same cult as Aelyn. Volatile and very angry about being passed up as leader - he will meet Chaos for himself and have his word be law.

El

Secondary doctor to Monty's primary position and lives in a plague-ridden town that barely has anyone left. There's a lot about El that he doesn't want people to know, but boy he do love him wife.

Eva

Prodigy mechanic and automaton maker that lives in Icarus with her adoptive mother. Creator of Ferro and Dog - though people are not sure how she was able to make her personal automatons so much more advanced than normal.

Ferro & Dog

Eva's most advanced creations - to the point of them being considered sentient. This is due to Eva's magic. Dog has had to be "reupped" a couple of different times. Losing all the training and memories from before each time. Ferro is far more advanced and hasn't had to be "reupped" yet. Though he is terrified for when that happens as he doesn't want to lose himself.

Julius

The head of the Wings of Icarus and acts as chaperone to the group to make sure they do what they're supposed to do. Frequently bickers with Markus.

Lila (A)

Eva's mirror twin. Was thrown into an asylum at four years old after an incident in the square where she broke down. With no one to claim her that is where she has stayed for the past decade. Reportedly.

Lucy

El's wife and former asylum inmate before the big breakout. Very proper, very calculating, very unnerving.

Magdelena

Holds herself very poised, but coiled like a snake. The adoptive mother of Eva and dragged into things by her ex-lover, Markus. Supplies Eva with metals to use when she can due to her own witchly magic.

Markus

Tall, attractive, and an absolute dillpickle. Markus is the one who accidentally unleashes Chaos after touching the jewel and names his ex - Maggie - as conspirator to drag her down with him. Ringleader of the traveling Circus Troupe/Thieves Ring. (I feel like it's important to note that Markus hasn't seen Maggie in near a decade.)

Monty

Sadness masked with a blanket of waning hope and determination that barely helps keep him alive. Primary doctor of a town that's slowly dying to the plague.

Scarlette

Rarely seen without her trademark red lipstick - Scarlette is another ex-lover of Maggie's due to a huge misunderstanding between the two of them. She cares so much for Eva (and only Eva, according to her) that she goes along to help the group so they don't die to the prison collars.

Sophia

Markus' confidant and acts as a knife-throwing tattooed woman in the circus. Was also caught in the theft, but was not the one who touched the jewel.

Vienna

Another one of Markus' troupe that was captured that night. A gremlin by nature, she uses her small form and high energy to use as a grave robber and pickpocket. Has a problem with the drink.

Taglist: @satohqbanana || @thebejeweledwatercat || @steh-lar-uh-nuhs || @drawnecromancy

[cherenkovs]

oh my god fml...i got my station all set up to start my new fibroblasts...sterilized the incubator...organized the shelves and stocked new disposables... go down to the -80 to get the cells...NEVER ORDERED THEM!!!!!!!!!

[bumblingwitch]

Ya'll want a large and diverse cast of various LGTBQ+ Identities? Ya'll want to see witches, plague doctors, cults, and steampunk cities?

Ya'll want to see the main storytelling device be a train? (cause we all fucking love trains here lbr.)

What about messy relationships?

What about people who are NOT good people, but deserve to live anyway?

What about seeing the most cringefail, poor little meow meow that I have literally EVER come up with?

THEN TELL ME TO WRITE THE DAMN THING ALREADY HOOLY SHIT.

[birdsean]

If I could go to any race in history it would probably be Macau 91, Only time the top gr.A teams raced each other. DTM vs JTC vs ATCC

[ask-the-creative-crew]

ATCC - Comeback Update ! 🎉

[mod: 🎩 we tip our hats to you, crew, for being so patient with all the inactivity, but it looks like we are finally ready to come back strong ! below are a few notes to take notice of before any new posts start rolling out:

☆ certain alterations have been made to some characters - any personality/physical changes going forward are completely intentional.

☆ a few references to the TV-show may be made, but characters from the TV-show are currently not available for asks ! (sorry !)

☆ the ask box will remain closed for now in order to get older asks answered first, however another announcement will be made for when it reopens. 📬

thank you for reading. see you soon ! ⭐️ ]

[hirocimacruiser]

"With the slogan of defeating the GT-R, the Supras are fighting for the title. With Gardner as the driver, it is attracting a lot of attention."

The Gardner the caption is referring to is of course Wayne Gardner who after winning the GP motorcycle World title switched to racing cars till his retirement from racing in 2002. He started in the ATCC before switching to racing one of the TOM'S JZA80 Supras in the JGTC.

[ilumel]

about me — [♡] ;

reyna / 22 / they

autistic ( among other things )

mutuals can ask for my toyhouse & oc blog <3

i'm very passionate about my interests & ccs!

i write a lot ( both original & fan fiction ) and also draw whenever my eternal art block lets me rest

i have a lot of ocs and will occasionally ramble about them! please feel free to ask about them or tell me about your own—i also really love making ocs with people!

relevant special interests are star wars, dragon age, destiny, & mass effect!

professional crow destiny enjoyer <3

always feel free to come chat or infodump to me!! i am also happy to play destiny with you if we're friends <3

tag directory — [♡] ;

#fave ( favorite posts )

#atcc ( comfort characters )

#my art ( self explanatory )

#.txt ( original textposts )

#mine ( misc original posts )

links — [♡] ;

carrd ( remaking ) / ask for discord or bungie id!

[stargazerspringles]

“Your time is up”

ART, me has brain worms about my story [AtCC]. College is wack, art? No time for it woooo so me going ham for 6 hours straight? Hecc yeah!!!!

[wackysach]

Everyone knows that. 🏡📅

[iamnaturalnana]

Heard of HEK 293? (Human Embryonic Kidney Cells)

293 [HEK-293] (ATCC® CRL-1573™) Organism: Homo sapiens, human / Tissue: embryonic kidney / ~~~ HEK 293 cell lines Risk summary and guidelines for risk management https://biosafety.wsu.edu/hek-293-cell-lines/ ~~~ What You Don’t Know About Flavor Enhancers Can Harm You https://articles.mercola.com/sites/articles/archive/2011/09/01/what-you-dont-know-about-flavor-enhancers-can-harm-you.aspx

[literarynecromancy]

Icarus Fact #1

The major trade goods that are produced in Icarus are jewelry and other fine things. But as of late there has been a shift to the production and selling of automative servants called Automatons - which are all the rage even outside of Icarus.

-"The Cogs of Icarus", Cleo the Nighttime Scribe

Taglist: @satohqbanana || @watermeezer || @steh-lar-uh-nuhs | @drawnecromancy

[oaresearchpaper]

Molecular Characterization of Aspergillus flavus in Imported Maize in Kenya

Abstract

Maize is a vital staple crop in Kenya, serving as a primary source of food and feed. Contamination of maize (Zea mays) by Aspergillus flavus  and the subsequent production of aflatoxins pose significant threats to food safety and human health. The risk of A. flavus contamination on imported maize at both gazetted and un-gazetted points of entry has not been extensively studied. The primary objective of this study was to examine the genotypic, phenotypic, and aflatoxigenic traits of A. flavus biovars derived from imported maize at Gazetted and Un-gazetted Points of Entries in Kenya. Furthermore, the study sought to establish the phylogenetic relationships among the identified A. flavus strains. A total of 600 imported maize samples were tested for aflatoxin contamination using the Total aflatoxin ELISA test. Out of 600 samples, 4.17% tested positive and were further subjected to morphological and molecular studies.  The morphological analysis revealed the presence of 13 biovars of A. flavus. Micro-morphologically, variations were observed in spore color, size, structure, conidiophore structure, and vesicle shape. The specific primers Calmodulin (CaM), the ITS1-5.8S-ITS2 region of the ribosomal DNA was successfully amplified in 10 out of the 13 biovars that were presumed to be A. flavus, confirming their positive identification as A. flavus. A single band of approximately 700 bp, which corresponds to the expected size of the ITS region in Aspergillus flavus, was observed in 10 out of the 13 biovars. This indicates the presence of A. flavus DNA in those biovars. The amplification of the ITS region provides a specific molecular marker for the identification of A. flavus. These findings highlight the significance of aflQ (ordA) and aflD (nor-1) genes as reliable markers for evaluating the aflatoxigenic potential of A. flavus biovars. Regarding aflatoxigenicity, DV-AM   method was used, and qualitative analysis was conducted. Out of the 13 biovars of A. flavus biovars tested, 23.08% exhibited aflatoxigenicity, while the remaining 10 biovars did not show any aflatoxigenicity. These findings indicate the presence of both aflatoxigenic and non-aflatoxigenic strains of A. flavus among the imported maize samples. The phylogenetic analysis revealed that Taxon 31 (AY495945.1 Aspergillus flavus biovar 92016f aflR-aflJ intergenic region partial sequence) and Taxon 32 (NR 111041.1 Aspergillus flavus ATCC 16883 ITS region from TYPE material). This genotypic and phenotypic characterization provides valuable information for understanding the diversity and potential toxigenicity of A. flavus strains on imported maize. This study contributes to the understanding of the genotypic and phenotypic characteristics of A. flavus on imported maize in Kenya.

Introduction

Maize plays a central role in the food security and livelihoods of Kenyan populations. It serves as a staple food crop for a significant portion of the population, contributing to both dietary needs and income generation. Moreover, maize is an essential component of livestock feed, supporting the growth of the domestic livestock industry. In sub-Saharan Africa as a whole, maize is ranked third in importance among cereal crops, following rice and wheat (Shiferaw et al., 2011). The cultivation and trade of maize have a considerable impact on regional economies and food systems. Maize (Zea mays) is often contaminated by Aspergillus fungal species during pre- and post-harvest practices, storage, and transportation. Studies by Horn (2007) showed that Aspergillus species are commonly found in the soil, which acts as a source of primary inoculum for infecting developing maize kernels during the growing season. Aspergillus flavus is distributed globally with a high frequency of occurrence in warm climates which favor the growth of the fungus (Cotty et al., 1994).

Understanding the population structure and genetic diversity of A. flavus is crucial for diversification of effective management strategies. Different strains of A. flavus may have varying levels of aflatoxin production and pathogenicity, which can influence the severity of contamination in maize (Abbas et al., 2013). Additionally, certain strains may exhibit resistance or susceptibility to control measures, such as biological control agents or fungicides. Therefore, identifying specific strains or groups within the A. flavus population can aid in the selection of appropriate control strategies to minimize aflatoxin contamination. Moreover, the genetic diversity of A. flavus may also have implications for host-pathogen interactions and disease development. Different strains may exhibit variations in their ability to infect maize kernels, colonize host tissues, and compete with other microorganisms in the maize ecosystem (Atehnkeng et al., 2014). Understanding these interactions can help in the development of resistant maize varieties and cultural practices that can limit fungal growth and subsequent aflatoxin production. The population structure and genetic diversity of A. flavus strains isolated from maize play a significant role in aflatoxin contamination and disease development. The existence of multiple strains within the A. flavus population highlights the need for comprehensive investigations to characterize their phenotypic and genotypic traits. Such studies will provide insights into the factors influencing aflatoxin production, the design of effective control strategies, and the development of resistant maize varieties to minimize the health and economic risks associated with aflatoxin contamination. Aspergillus species, including Aspergillus flavus, are of great concern due to their ability to produce aflatoxins, potent carcinogens and toxins that contaminate various agricultural commodities, including maize. The accurate identification and characterization of Aspergillus species is crucial for assessing their potential to produce aflatoxins and understanding their impact on food safety.

Gene sequencing has emerged as a powerful tool for the accurate identification and classification of Aspergillus species. In recent years, numerous studies have utilized gene sequencing data to characterize Aspergillus biovars from different sources. By comparing the genetic sequences of specific genes, such as the internal transcribed spacer (ITS) region, researchers can determine the species and genetic diversity within a population. In addition to genetic characterization, a polyphasic approach is commonly employed to identify and characterize Aspergillus biovars. This approach combines morphological and molecular analyses to provide a comprehensive understanding of the biovars. Morphological characteristics, such as colony color, texture, spore color, size and structure, conidiophore structure and vesicle shape are observed and recorded. These characteristics help in differentiating between various Aspergillus species and subgroups. Furthermore, molecular techniques, including polymerase chain reaction (PCR) amplification and sequencing of specific genetic markers, allow for a more precise identification of aflatoxigenic and nonaflatoxigenic A. flavus biovars. These methods target genes associated with aflatoxin production, such as the aflatoxin biosynthesis cluster genes, to determine the potential of a biovar to produce aflatoxins. The combination of gene sequencing and polyphasic approaches provides a comprehensive understanding of the genetic diversity, population structure, and aflatoxinproducing potential of Aspergillus species, particularly A. flavus. This information is essential for risk assessment, development of effective control strategies, and ensuring the safety and quality of imported maize and other agricultural commodities.

This study contributed to the understanding of the population dynamics and potential risks associated with A. flavus in imported maize. Given the prominence of maize in Kenya, research efforts focusing on this crop are crucial. The genotypic and phenotypic characterization of A. flavus on imported maize assumes particular significance in the Kenyan context. A thorough understanding of the genetic diversity and potential for mycotoxin production in A. flavus populations is essential for developing effective control strategies and mitigating the health risks associated with mycotoxin contamination. Gazetted and un-gazetted points of entry play a crucial role in facilitating the importation of maize. However, the risk of A. flavus contamination in imported maize has not been thoroughly investigated, warranting a comprehensive genotypic and phenotypic characterization of this fungus. Understanding the genotypic and phenotypic characteristics of A. flavus on imported maize is essential for several reasons. Firstly, it allows for the identification of specific genetic traits and phenotypic features associated with higher aflatoxin production, thus enabling the development of targeted control strategies. Secondly, it provides insights into the diversity of A. flavus biovars present in imported maize and their potential for aflatoxin contamination. This knowledge can contribute to risk assessment and management strategies aimed at preventing or minimizing aflatoxin contamination in the domestic maize supply chain.

Genotypic characterization involves studying the genetic makeup of A. flavus biovars to determine their relatedness, genetic diversity, and potential for toxin production. Several molecular techniques have been used for genotyping A. flavus, including random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and multilocus sequence typing (MLST) (Abdallah et al., 2018). These methods have provided valuable insights into the genetic diversity and population structure of A. flavus, highlighting the presence of distinct genotypes in different geographic regions (Klich et al., 2015). Phenotypic characterization involves studying the observable traits and behaviors of A. flavus, such as growth patterns, conidiation, and mycotoxin production. Phenotypic characterization is essential for understanding the pathogenicity and virulence of A. flavus strains on imported maize. Researchers have observed variations in colony morphology, growth rate, and sporulation among different A. flavus biovars (Calvo et al., 2016). Furthermore, studies have demonstrated the production of mycotoxins, particularly aflatoxins, by certain A. flavus strains (Chang et al., 2019). Phenotypic characterization provides valuable information for risk assessment and identifying high-risk A. flavus biovars in imported maize. The genotypic and phenotypic characterization of A. flavus on imported maize plays a crucial role in assessing the potential health risks associated with mycotoxin contamination. By combining genotypic and phenotypic data, researchers can identify highly toxigenic A. flavus strains and evaluate their prevalence in imported maize.

This information is essential for implementing targeted control measures, such as crop management strategies, post-harvest interventions, and storage practices, to minimize mycotoxin contamination and ensure food safety (Li et al., 2020). Investigating A. flavus on imported maize specifically at gazetted and ungazetted points of entry in Kenya is crucial. Gazetted points of entry are official border checkpoints designated for the importation of agricultural products, while un-gazetted points of entry refer to informal channels through which goods, including maize, are smuggled into the country. Analyzing both types of entry points can provide a comprehensive understanding of the risks associated with A. flavus contamination in imported maize, as well as the efficacy of control measures implemented at official checkpoints. In this study, we aim to conduct a detailed genotypic and phenotypic characterization of A. flavus on imported maize at both gazetted and un-gazetted points of entry in Kenya. We will analyze the genetic diversity, aflatoxin production capability, and other phenotypic traits of A. flavus biovars obtained from imported maize samples. By doing so, we hope to gain insights into the potential sources and pathways of A. flavus contamination in imported maize and develop targeted strategies to ensure the safety and quality of imported maize in Kenya.

Source :  Molecular Characterization of Aspergillus flavus in Imported Maize in Kenya | InformativeBD

[healthcaremarketanalysis]

Cell Dissociation Market worth $1.4 billion by 2028

The global cell dissociation market in terms of revenue was estimated to be worth $0.6 billion in 2023 and is poised to reach $1.4 billion by 2028, growing at a CAGR of 17.8% from 2023 to 2028.

Download PDF Brochure:

Cell dissociation Market Dynamics

Driver: Increase in recombinant therapeutics sourced from mammalian cells

The biopharmaceutical industry has witnessed a considerable increase in the proportion of recombinant therapeutics sourced from mammalian cells since 2002. The number of approved recombinant products from mammalian cell culture increased by 8.5% annually from 2002 to 2022. As of June 2022, more than 300 recombinant products have been commercialized, indicating a rapid shift towards mammalian-based therapeutics, which nearly account for 67% of therapeutics in 2022. .Cell culture products, including cell dissociation form an integral part of developing mammalian-based recombinant therapeutics, propelling the industry growth.

Restraint: High cost of cell-based research

Isolation and purification of cell organelles integrates expensive products, additionally, automated/sem-automated benchtop instruments are more expensive than traditional methods. Despite significant benefits offered by automated instruments, high costs associated with these instruments may limit the adoption of products further restraining the market growth.

Opportunity: Advancements in non-enzymatic tissue dissociation

Companies operating in the market are introducing innovative and advanced products to overcome competition and generate more revenue. Most researchers in R&D institutes and biotechnology companies have primarily relied on traditional enzymatic dissociation products to separate cells from primary tissues. Although these traditional enzymatic dissociation products have advantages, such as better dissociation while performing research, they can sometimes be cytotoxic and damage viable cells. To overcome this issue and differentiate their product offerings, several companies are introducing non-enzymatic products that are non-cytotoxic. Owing to this advantage, the demand for non-enzymatic dissociation products is increasing among several end users.

Challenge: Limitations associated with dissociated cell culture

The key limitation of dissociated cultures is the small number of cells relative to immortalized cell lines. This makes it difficult to perform biochemical experiments requiring a high starting material volume. Additionally, most primary cell cultures are not homogeneous, which poses a challenging scenario for cell dissociation experiments. Neuronal cultures are often mixtures of both glia and neurons that respond to different neurotransmitters, so identifying an individual population of cells can be difficult.

North America dominates the global cell dissociation market

The cell dissociation market is segmented into five major regional segments, namely, North America, Europe, Asia Pacific, Latin America, and Middle East & Africa. In 2022, North America accounted for the largest share in the cell dissociation market, which is attributed to increasing investments in the development of novel cell-based therapies.

The cell dissociation market is moderately consolidated in nature with prominent market players such as Merck KGaA (Germany), Danaher Corporation (US), Thermo Fisher Scientific, Inc. (US), Corning Incorporated (US), Becton, Dickinson and Company (US), STEMCELL Technologies (Canada), PromoCell GmbH (Germany), Miltenyi Biotec (Germany), ATCC (US), HiMedia Laboratories (India), PAN-Biotech (Germany), CellSystems GmbH (Germany), AMSBIO (England), Neuromics (US), VitaCyte, LLC. (US), ALSTEM (US), Biological Industries (Israel), Gemini Bio (US), Innovative Cell Technologies, Inc. (US), Central Drug House (P) Ltd. (India), Worthington Biochemical Corporation (US), Capricorn Scientific (Germany), Abeomics (US), and Genlantis, Inc. (US).

Recent Development of Cell Dissociation Industry

In 2020, Miltenyi Biotec launched automated and closed adherent cell culture solutions on CliniMACS Prodigy.

In 2020, Merck The company expanded its Life Sciences production facilities in Danvers, Massachusetts, and Jaffrey, New Hampshire (US).

In 2020, STEMCELL Technologies partnered with CollPlant to secure CollPlant rhCollagen for STEMCELL’s use in cell culture applications.

In 2021, Danaher (Cytiva) partnered with Diamyd Medical, wherein Diamyd selected Cytiva’s FlexFactory platform for making precision medicine type-1 diabetes vaccines.

In 2021, Danaher (Cytiva) collaborated with the Government of Telangana (India) to strengthen the biopharma industry in India with new labs.

Cell Dissociation Market Advantages:

Increased Efficiency: Cell dissociation reagents provide a more efficient and reliable method for dissociating cell samples, which can significantly reduce processing time and improve the quality of the results.

Higher Yields: Cell dissociation reagents are designed to maximize cell yield, allowing for more effective downstream applications such as flow cytometry and immunological assays.

Improved Quality: Cell dissociation reagents help to ensure that cells are undamaged during the isolation process, thereby resulting in higher quality samples.

Cost Effective: Cell dissociation reagents provide a cost-effective solution, as they are typically less expensive than traditional methods.

Greater Flexibility: Cell dissociation reagents offer greater flexibility in terms of the types of cells that can be isolated, allowing for a wider range of downstream applications.

Related links:

[birdsean]

They added the Dick Johnson atcc mustang in gta.