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qocsuing · 5 months ago

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IP2World: A Leading Provider of Residential IP Proxy Services

IP2World: A Leading Provider of Residential IP Proxy Services In the ever-evolving digital landscape, maintaining online privacy and security is more crucial than ever. Proxy servers have become indispensable tools for individuals and businesses alike, offering a range of benefits from anonymous browsing to data scraping. Among the myriad of proxy service providers, IP2World stands out as a leading provider of residential IP proxy services. This article explores the features, advantages, and applications of IP2World’s offerings, providing a comprehensive overview for potential users.To get more news about ip2world.com, you can visit ip2world.com official website.

IP2World boasts an impressive pool of over 90 million residential IPs. This extensive network ensures that users can access a diverse range of IP addresses, enhancing their ability to bypass geo-restrictions and avoid detection by websites.

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With coverage in over 220 locations worldwide, IP2World provides users with the flexibility to choose IP addresses from specific countries, cities, or even ASNs (Autonomous System Numbers). This global reach is particularly beneficial for businesses that require localized data or need to test their services in different regions.

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aayushi1806 · 2 years ago

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What Is Papain Enzyme And How Is It Made?

Overview Of Papain Enzyme

Papain, also known as papaya proteinase I, is a��cysteine protease (EC 3.4.22.2) enzyme present in papaya (Carica papaya) and mountain papaya (Vasconcellea cundinamarcensis). Papain is taken from the fruit of the papaya tree. It is used to make medicine.

Some people take papain by mouth for pain and swelling (inflammation) and to remove extra fluid following trauma and surgery. It is also taken by mouth to help with digestion, to treat parasitic worms, inflammation of the throat and pharynx, shingles (herpes zoster) symptoms, sore muscles, diarrhea, hay fever, runny nose, and a skin condition called psoriasis. Papain is also taken by mouth to treat the side effects of radiation therapy, or it may be used in combination with other therapies to treat tumors.

Some people apply papain directly to the skin to treat insect or animal bites, infected wounds, sores, and ulcers. In manufacturing, papain is used in cosmetics, toothpaste, contact lens cleaners, meat tenderizers, and meat products.

Structure Of Papain Enzyme

The papain precursor protein contains 345 amino acid residues and consists of a signal sequence (1-18), a propeptide (19-133) and the mature peptide (134-345). The amino acid numbers are based on the mature peptide. The protein is stabilised by three disulfide bridges. Its three-dimensional structure consists of two distinct structural domains with a cleft between them. This cleft contains the active site, which contains a catalytic diad that has been likened to the catalytic triad of chymotrypsin. The catalytic triad is made up of the amino acids – cysteine-25 (from which it gets its classification) and histidine-159. Aspartate-158 was thought to play a role analogous to the role of aspartate in the serine protease catalytic triad, but that has since been disproved.

Function Of Papain Enzyme

The mechanism by which papain breaks peptide bonds involves the use of a catalytic triad with a deprotonated cysteine. Asn-175 helps to orient the imidazole ring of His-159 to allow it to deprotonate the catalytic Cys-25. This cysteine then performs a nucleophilic attack on the carbonyl carbon of a peptide backbone. This forms a covalent acyl-enzyme intermediate and frees the amino terminus of the peptide. The enzyme is deacylated by a water molecule and releases the carboxyl-terminal portion of the peptide. In immunology, papain is known to cleave the Fc (crystallisable) portion of immunoglobulins (antibodies) from the Fab (antigen-binding) portion.

Papain is a relatively heat-resistant enzyme, with an optimal temperature range of 60 and 70 °C. Papain prefers to cleave at (hydrophobic)- (Arg or Lys)- cleaves here – (not Val). Hydrophobic is Ala, Val, Leu, Ile, Phe, Trp, or Tyr.

Uses Of Papain Enzyme

Papain breaks down tough meat fibers and has been used for thousands of years to tenderise meat eaten in its native South America. Meat tenderisers in powder form with papain as an active component are widely sold.

Papain can be used to dissociate cells in the first step of cell culture preparations. A ten-minute treatment of small tissue pieces (less than 1 mm cubed) will allow papain to begin cleaving the extracellular matrix molecules holding the cells together. After ten minutes, the tissue should be treated with a protease inhibitor solution to stop the protease action. Left untreated, papain activity will lead to complete lysis of the cells. The tissue must then be triturated (passed quickly up and down through a Pasteur pipette) to break up the pieces of tissue into a single cell suspension.

It is also used as an ingredient in various enzymatic debriding preparations, notably Accuzyme. These are used in the care of some chronic wounds to clean up dead tissue.

Papain is added to some toothpaste and mint sweets as a tooth whitener. Its whitening effect is, however, minimal because the papain is present in low concentrations and is quickly diluted by saliva. It would take several months of use to have a noticeable effect.

Papain is the main ingredient of Papacarie, a gel used for chemomechanical dental caries removal. It does not require drilling and does not interfere in the bond strength of restorative materials to dentin.

Papain has been known to interfere with urine drug tests for cannabinoids. It is found in some drug detox products.

Recently, it has been demonstrated that papain can be used to assemble thin films of titania used in photovoltaic cells.

Benefits Of Papain Enzyme

1. Stimulates Digestion

One of the key areas in which papain serves the body is in the realm of its protein-digestive properties. One case study found that when a male patient with gluten intolerance ate a gluten-free diet, he still experienced diarrhea, but when he additionally took 1800 mg of papain for one month, he had fewer loose stools and less malabsorption. This is just one study, and more research needs to be conducted.

2. Aids Skin and Wound Healing

Due to papain’s beneficial capacities, people have used it for many years as a topical application to burns, ulcers, irritations, bedsores and other wounds, and to assist recovery from sports injuries. Some practitioners have used it dental cavities. Papain’s enzymatic action is very specific, and it does not harm healthy skin. Traditional cultures in Hawaii and Tahiti made poultices out of the skins of papaya, as this part of the fruit has a particularly high concentration of papain. Traditional healers applied this substance to the skin to heal wounds, burns, rashes and insect stings.

3. Digests Mucus

Studies have found that papain digests sinus mucin, a glycoprotein found in mucus, and hence may have beneficial effects for people having sinus issues. Papain makes mucus less viscous, or runnier, and hence better able to be eliminated. Because of this feature, some researchers are studying how papain can help deliver nanoparticle medicines to the body so that they can get through the body’s natural mucosal barrier in the gut. Using papain with nanoparticles may not be the best for your health.

4. Supports Immune System Function

Studies have found that papain may have anti-cell proliferation properties. Some studies have shown papain delivers a strong effect while others found no difference between papain and controls. A review article found strong evidence for the overall immune function properties of papaya.

5. Resists Redness and Irritation

Studies confirm that the papain enzyme offers powerful resistance to redness and irritation. Papain helps aid the absorption of another beneficial substance, quercetin. One study found that when papain and bromelain were given along with quercetin, it helped swelling symptoms associated with prostate health.

6. Acts as an Antioxidant

Papain holds compounds that may aid in protecting the body from cellular damage caused by free radicals, which makes it an antioxidant. The compounds in papaya juice effectively scavenge, or counteract, highly reactive hydroxyl (OH-) free radicals, as well as super-oxides. Papain has an antioxidant level on par with Vitamins E and C. In one study, the Sunrise Solo cultivar (a type of papaya) was more effective as an antioxidant than two other cultivars.

7. Prevents Food Spoilage

Since research has shown papain has antifungal and antibacterial properties, and it is sometimes used to preserve foods naturally. It is a powerful agent commonly used in food preservation, reducing bacterial infestations and spoilage due to oxidation.

How Does Papain Tenderize Meat?

The most important characteristics of meat quality are tenderness, juiciness, and flavour. Consumers consider tenderness as the most important factor in determining eating satisfaction of beef (Issanchou, 1996, Boleman, Miller, Taylor, Cross, Wheeler, Koohmaraie, Johnson and Savell, 1997). Tenderness is defined as the ease of mastication, which involves the initial ease of penetration by the teeth, the ease with which the meat breaks into fragments and the amount of residue remaining after mastication (Lawrie, 1998).

Meat tenderness depends on spices, breed, age, sex and individual skeletal muscle tissue of the animal. Tenderness originates in structural and biochemical properties of scheletal muscle fibers, especially myofibrils and intermediate filaments, and of the intramuscular connective tissue, the endomysium and perimysium, which are composed of collagen fibrils and fibers (Takahashi, 1996). The mechanical stability of collagen fibrils increased markedly with chronological aging.

Meat produced from an old animal is tough and has a lower eating quality. Improvement of meat tenderness of aged cattle is necessary to increase functional properties and value of the meat (Shiba, 2004). Meat can be tenderized in different ways: mechanical methods (mincing or hitting to crush the conjunctive and muscle tissue), chemical methods by injecting in the muscle solutions with chemical substances (salt, sodium chloride, sodium polyphosphate, potassium lactate, sodium diacetate all dissolved into water, R.K., 2003), enzymatic methods used proteolytic enzymes like papain, bromelain or ficin.

Enzymatic tenderisation can be made in a humid way (by injecting in the muscle solutions with enzymatic preparations) or in a dry way (pressing tender mixtures on the meat surface). The end of the tenderisation process and the method employed for aging and thermal processing should be established to get an optimum sensorial quality of the meat.

Injection of beef cuts with papain cause an important improvement of functional properties of adult beef. Papain is a powerful proteases preparation, with great under-layer specificity, catalysing the breaking of the peptidic bonds in the protein molecules and their degradation products to amino acids. The increase of the papain level, from injection brine, as well as the increase of the time of ageing, determined a significant weakening of the meat structure. Papain tenderisation of the adult beef determined the improvement of tenderness, flavour, and juiciness. It is recommended to use papain doses as low as possible, in order to avoid advanced tenderisation and obtaining meats with soft structure, with a very low resistance to mastication and paste texture. Application of this technology could assist beef producers and processors to obtain meat products that can satisfy consumer’s expectation.

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abdulhannanmannanhira · 2 years ago

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Warehousing and Fulfillment Companies

Fulfillment.com offers turnkey services for E-commerce retail and noncommercial order fulfillment. By simply transferring Fulfillment.com a list of product SKUs, also integrating their CRM, our guests are just way down from going live with a crusade.Fulfillment.com makes you functional in nearly no time and our adaptable technology model can accommodate rapid-fire volume increases as fluently as steady, diurnal order growth. Stylish of all, you can take advantage of our volume shipping abatements anyhow of the volumes you're dispatching.

Numerous companies specialize in one or two areas of logistics and warehouse fulfillment services, but at FDC, we give a comprehensive suite of services at each of our global storages. From well- defined entering parameters for dispatching your force to an Fulfillment.com installation, to a stoner-friendly dashboard walk- through with your devoted Account director, to order fulfillment, supplementary crusade operation, and indeed returns processing,Fulfillment.com adroitly manages your business’s order fulfillment needs. FDC’s entering platoon spells out the exact conditions for entering your payload at an Fulfillment.com fulfillment installation. These warehousing exponents aim to unload a pallet in 15 twinkles or lower, checking force against the quilting list and/ or Advanced Shipping Notice( ASN), pulling QA samples as directed by you, and allocating your product to its specific, designated warehouse position.

Opting the Right Fulfillment installation

FDC’s technology tools streamline order uploads via CRM orE-commerce platform integration, CSV upload, or indeed homemade order input via our system interface, furnishing real time reporting and force tracing24/7. FDC’s system creates an dispatch alert once a day including force situations and anticipated reduction rates grounded on current orders and shipping volume statistics. When our Returns department receives returned product, they perform a detailed visual check of all unopened, undamaged products to determine whether or not the product can be returned toinventory.However, it's returned to the system and incontinently viewable in force via our dashboard, If the product is respectable. guests can get an overview of their account exertion at any time, penetrating data on force, products packed , orders grouped, product returns, postage costs, and more.

How Fulfillment storages Differ ?

When completingE-commerce orders, attention to detail is consummate and FDC’s pick, pack, and boat system is impeccably suited to match this need. FDC’s platoon provides a variety of supplemental fulfillment services, from same day order fulfillment, to completion of roster andE-commerce orders, to patron recognition deliveries on crowd- source funded juggernauts, to delivery of promotional and ultraexpensive papers, to subscription services including issue inserts, poly- bagging, and distributions for contest winners. fresh services number complete product assembly and kitting services, including collectively bubble- wrapping fragile particulars and grouping separate product particulars into one, technical package; product labeling; palletco-packing and shrink wrapping; and pallet break down andre-wrapping. FDC’s installations are operationally optimized for the rapid-fire processing of small parcels. By allowing Fulfillment.com to manage your business, we can help you grow your request share, expand your transnational reach, and strengthen your business brand, all while contributing to your nethermost line.

#Warehouse

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advancedsolutionsnederland · 3 years ago

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ASN consultancy overview

Get a detailed overview of ASN's applied and integrated algorithms solutions for public infrastructure, IoT security, Smart cities, biomedical and more...

#iot #smartcities #biomedical #engineering #sensors #Youtube

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felonyring73 · 4 years ago

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Peptide

What Is Sarm? Every Little Thing You Need To Understand

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Product Classifications.

Sarm (sarm Rabbit Polyclonal Antibody.

Journal Of Peptide Scientific Research.

Procollagen Peptide Kind 3 (p3np Or Piiinp).

Manufacturing of BAPs by Lactobacillus types is relatively inexpensive, compared to making use of cleansed enzymes. It additionally has a better potential to generate brand-new peptide series as well as, therefore, brand-new BAPs with particular biological homes. Nevertheless, there are still some challenges for further commercial exploitation of lactobacilli, such as the poor characterization of CEPs from various species and also the low returns of peptide production by fermentation. proven sarms of the proteolytic system of lactobacilli can overcome these difficulties. In this evaluation, an approach was suggested for selecting efficient BAP-producing Lactobacillus stress.

How long is LGD 4033 in your system?

While clinical trial participants on Ligandrol did have a higher rate of throat infections, it was concluded this was not due to the drug. Ligandrol can be detected for up to 21 days in the urine of those who take it.

The comprehensive discussion on the hidden devices as well as effects of microbial resistance to AMPs is past the range of this review, as well as the viewers is described the outstanding current evaluation by Andersson et al. Significantly, it is suggested that bacterial fatality triggered by AMPs might be a result of multiple and also complementary actions, described as multi-hit mechanism. Peptides standing for the 3 major classifications of the second structures of AMPs.

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Vitamin C has additionally been revealed to assist bone recovery, which may be related to the function this plays in synthesizing collagen in our body. There is additionally some proof that collagen supplements might help in the therapy of rheumatoid joint inflammation; which is an inflammatory form of arthritis that causes swelling as well as pain in the joints. However this evidence is combined, as well as there is a lot stronger evidence to support well-known medical treatments for rheumatoid arthritis, such as the drug methotrexate. Collagen is a vital component of the connective tissue as well as cartilage material which is found in our joints. Some researchers have suggested that collagen supplements might aid in the therapy as well as prevention of osteo arthritis by minimizing swelling and preserving cartilage. Collagen is a protein which is naturally created in our body.

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oligosaccharide, polysaccharide, or their by-products (e.g. sulfo- or phospho-substituted). Proteoglycans are a subclass of glycoproteins in which the carbohydrate units are polysaccharides that contain amino sugars.

Sarm (sarm Bunny Polyclonal Antibody.

The chain of repeating systems is connected to the protein by an oligosaccharide of a structure different from the duplicating devices. This linkage area is identical in the majority of the proteoglycans, but is different in keratan sulfate. Buy SARMs contains repeating systems of N-acetyllactosamine that are O-sulfated. Proteoglycans may additionally have one or more oligosaccharides of frameworks similar to those found in various other glycoproteins. The carboxyl group of the muramic acid is generally substituted by a peptide including residues of both L- as well as D-amino acids, whereas that of L-talosaminuronic acid is replaced by a peptide containing L-amino acids only. A glycoprotein is a compound containing carbohydrate covalently linked to healthy protein. The carbohydrate may remain in the kind of a monosaccharide, disaccharide.

What Are SARMs? – Types, Cycle, Benefits - - VENTS Magazine

What Are SARMs? – Types, Cycle, Benefits -.

Posted: Sun, 20 Oct 2019 07:00:00 GMT [source]

It is also one of the most bountiful protein in the body-- audit for about 30% of our overall healthy protein content. Collagen is a vital part of connective tissues-- which supply support, structure as well as security in our body, in addition to binding together organs or various other cells. Nonetheless, they have a tendency to glob with each other away from the polar or charged molecules. This clumping of hydrophobic particles is called hydrophobic communication. The coiling of RNA chain onto itself happens through hydrogen bonding. Hydrogen bond is formed as between a hydrogen atom and also a very electronegative atom like oxygen or nitrogen. When a hydrogen atom comes within the electron fondness of an extremely electronegative atom like oxygen or nitrogen, electrostatic forces of tourist attraction among the proton of hydrogen and also the lone pair of electrons in oxygen or nitrogen.

Journal Of Peptide Scientific Research.

For example, poultry ovalbumin consists of a solitary glycosylated amino-acid residue (Asn-293), yet more than a loads different oligosaccharides have been identified at that website. In proteoglycans, private glycosaminoglycan chains might vary in framework, e.g. the degree of sulfation, the proportion of glucuronic acid to L-iduronic acid, and chain size. Proteoglycans are for that reason very polydisperse, unlike common glycoproteins which, even with their microheterogeneity, are not markedly polydisperse in their molecular dimension. A proteoglycan is a protein glycosylated by one or more glycosaminoglycans. The hexosamines are usually N-acetylated, as well as in some of the glycosaminoglycans the D- glucosamine is N-sulfated. Varying levels of sulfation occur in other placements of the hexosamines as well as on the L-iduronic acid.

This choice treatment integrates using a conventional technique with targeted approaches based on in silico evaluation that were so far primarily utilized for BAPs produced by detoxified enzymes or gastro-intestinal enzymes. helveticus of dairy origin, 6, 4, 21, and also 20 of them had 4, 3, 2, or 1 CEP paralogs, respectively. Miyamoto et al. studied 6 pressures separated in Mongolia as well as reported various healthy protein hydrolysis patterns than in strains isolated in European or North American nations. The only paralog detected in the 6 Mongolian stress was PrtH1. Hydrolysis pattern distinctions were likewise observed between various Lb.

Procollagen Peptide Type 3 (p3np Or Piiinp).

Loaded with OG hydrating active ingredients, this serum will certainly leave your skin moistened AF. " At Sykea we believe your skin care must be as great for the planet as it is for your skin." Marine algae-based, vegan and cruelty-free skin care formulated utilizing extremely effective active ingredients for the ideal night and day Skin care routine.

In spite of the substantial development in our understanding of the incident, biosynthesis, residential properties and functions of the glycoproteins, this definition stays proper. Hereditary characterization of a cell envelope-associated proteinase from Lactobacillus helveticus CNRZ32. Characterization of the cell wall-bound proteinase of Lactobacillus casei HN14. A testimonial of QSAR researches to predict task of ACE peptide preventions.

Bioactive Healthy Protein as well as Peptides Market report evaluations the effect of Coronavirus (COVID-19) on the Bioactive Protein and Peptides sector. Because the COVID-19 infection break out in December 2019, the condition has actually infected virtually 180+ nations around the globe with the World Wellness Company declaring it a public wellness emergency situation. The degrees gauged are made use of to guide professional therapy as well as would certainly be examined along with investigations such as echocardiogram as well as heart MRI scan results. A couple of tiny researches have located that eating collagen, in the type of gelatin, might result in a decrease in hunger. It deserves noting that each of these studies consisted of much less than 25 individuals, and none gauged real changes in weight.

The most potent SARM for promoting one of the most muscle development is RAD 140, additionally known as Testolone.

For example, LGD-4033 and also S-23 have been carried out to balance out the bulking impacts of testolone.

LGD-4033 and also S-4 have been piled for their results on weight management, as have LGD-4033 as well as SR9009 Stenabolic and also GW501516.

It's also taken into consideration better in a stack with ostarine to assist increase healing time as well as keep up with the efficiency enhancement of testolone.

It boosts endurance and also strength, adds to the advancement of dry, compacted muscle mass.

Unscrupulous suppliers may market such products as "lawful steroids" or "steroid alternatives".

LL-37 and human lactoferricin represent α-helical peptides, human β-defensin 1 stands for β-sheet peptides, and indolocidin represents extended/random-coil frameworks. AMPs in nature are generated either by ribosomal translation of mRNA or by nonribosomal peptide synthesis. While nonribosomally synthesized peptides are generally produced by microorganisms, the ribosomally synthesized AMPs are genetically inscribed and produced by all varieties of life, bacteria included. Without effort to offer an extensive overview when it come to all kinds of AMPs identified from various resources, this review focuses on used therapeutic facets with the emphasis of AMPs being examined as prospective medicinal agents. xClose dialog boxPlease log in to your account to include items to your favourites.

This approach uses a mix of traditional methods and in silico devices. By describing various methods for BAP production and a method for pressure selection, this work aims at providing a basic operations for development of different BAP-containing products making use of lactobacilli. For instance, various CEPs were isolated as well as purified or partially purified from Pound. Relating to the production of BAPs using Lactobacillus species, the inter- as well as intra-genus variability of proteolytic activity and also specificity make stress option hard. A technique for choice of BAP-producing Lactobacillus strains is suggested.

Never ever lack your favourite Boots products with our worldwide shipment options. Sarm sarms.com to moisten the much deeper layers of the skin and has effective restoring homes. Penetrates the top layers of the skin and also helps to smooth out great lines. Stands up to 1000 times its weight in water developing a plumping impact while it likewise forms a moisturizing movie on the surface of the skin for noticeable, surface-level hydration. Use with Thirst Trap Cocoon Mask for mins as well as enable essence to fully soak up into skin. When almost completely dry, apply Thirst Catch Juice HA3 & Peptide Serum generously throughout your face and décolleté. Not only will this serum leave your skin literally drippin', but likewise firm, glowy as well as luminescent.

A certain glycoprotein might happen in kinds that vary in the framework of several of its carb units, a sensation understood microheterogeneity. Such distinctions may impact both the size and charge of specific glycoproteins; periodically, the differences might be only due to changes in link placement in a carb device.

Are we killing ourselves to look beautiful? - ABC Online

Are we killing ourselves to look beautiful?.

Posted: Sun, 15 Apr 2018 07:00:00 GMT [source]

Endonucleases, they can damage the phosphodiester bond also from within the chain of nucleotides. Exonucleases, they break the phosphodiester bond starting from one end of the chain. An example of thioester link is the one in between the thiol team of CoA and also the carboxylic team of acetic acid in Acetyl CoA. It is a tweaked kind of ester link in which the oxygen bridge is made use of to connect a carbon atom with a sulfur atom. It is formed as a result of the reaction between the carboxylic group of one molecule as well as the thiol group (- SH) of an additional molecule.

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examtech-blog · 5 years ago

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1Z0-1045 Oracle Warehouse Management Cloud 2019 Implementation Essentials Exam

CertsWarrior Loves to Write & Provide the Valid Material to its Customer. Oracle Warehouse Management Cloud 2019 Implementation Essentials describes the Setup and System overview in which it builds a setup of System Integration Framework and Sequence counter, Barcode types and Printers. This certification also describes the Outbound Orders in which it demonstrate the Order types, create orders and Integrate orders with WMS and also focuses in Wave Template and execute Waves.

An Oracle Warehouse Management Cloud 2019 certified specialist has verified the knowledge required to Setup parent Child company hierarchy, inbound process, execute outbound orders and manage inventory. Candidates earning this certificate are able to create Web reports, Perform Picking, packing, cycle Count, Check-in and receive Loads and Appointments. It also provides up to date training and field experience. This certificate also describes the Parent-Child Company hierarchy and also focuses on User Menu Configuration.

Overview of the Exam:

This <a href="https://www.certswarrior.com/vendor/oracle/oracle-cloud/">Oracle Warehouse Management Cloud 2019 </a> Implementation Essentials describes the Setup and System overview in which it builds a setup of System Integration Framework and Sequence counter, Barcode types and Printers. This certification also describes the Outbound Orders in which it demonstrate the Order types, create orders and Integrate orders with WMS and also focuses in Wave Template and execute Waves. Candidates through this certification learn to perform Packing, Picking and manage LTL Load and Shipping. This certification also configures a parcel Manifest and Loading and Shipping the Parcel manifests. This certification also describes the Web Reports in which it explains the web reports, Create Express, Standard, Crosstab and Dashboard Reports. This certification also validates that how to execute schedule and edit the reports. This certification not only defines the setup and system overview and outbound orders but also focuses on Setting up Inbound Processes in which candidates learn that how to create Purchase Orders and integrate them with Warehouse Management System (WMS).

Exam Topic Distribution: This certification also demonstrates that how to create Advanced Shipment Notifications (ASNs) and integrate them with WMS. It helps to check in a Load and receive by Load and create and check-in appointments. This certification helps to perform a receipt by ASN, with and without the RF and ASN verification. This certification describes the System Directed Quality Control (QC) and explains Cross Dock management and explains Inbound Sorting and performs Putaways. Oracle Warehouse Management Cloud 2019 Implementation Essentials certification also defines the Inventory Management in which it describes the Inventory Types and creates an item in Item master and integrate it with WMS. This certification also helps candidates in creating Locations in Location Master and describes different Inventory screens. Candidates learn about Comb/Split IB LPNs and execute a cycle count and explain Lot Management as this certification also focuses on Expiration Date and Receive Expiry date items. This certification also helps in tracking the serial numbers and configures and executes Replenishment.Nowadays, 1Z0-1045 oracle is gaining a lot of demands from IT industry. Many organizations are now placing this certification as the pre-requisite for their jobs. As a result, massive numbers of IT professionals are aiming to get Oracle Warehouse Management Cloud 2019 Implementation Essentials certification. Professionals like sales operations managers, customer service managers, service schedulers and administrators, executives, office managers and consultants who want to demonstrate a foundational understanding of the application functionality are seeking for the Oracle 1Z0-1045 certification.

Where to Find Training Courses? CertsWarrior is also providing online training courses, Study guides and preparation materials for the <a href="https://www.certswarrior.com/exam/1z0-1045/">1Z0-1045 Exam </a> and the information of these exam training materials are available at ORACLE CertsWarrior website. Sample practice or self-assessment tests are also available at ORACLE online for measuring the level of skills and knowledge.

Road to the Achievement by Using our Latest and Workable Study Material Regarding <a href="https://www.certswarrior.com/vendor/oracle/oracle-cloud/">ORACLE 1Z0-1045 </a>” Practice Test and PDF Questions.

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stlhandyman · 2 years ago

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leftcomputerpost · 3 years ago

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Glycoprotein Analysis Market Study: An Emerging Hint of Opportunity by 2031

Glycoprotein Analysis Market Overview

Glycoprotein is the proteins with oligosaccharide moieties which emerge after glycosylation during post-translational or co-translational modification of proteins.

Glycoprotein plays a crucial role in cellular metabolism and functions including cell signalling, cellular adhesion, immune system, pathogenicity, genesis and progression of metabolic disease and biomarker of diseases such as cancer.

There Glycosylations are of two types, O-glycosylation on Serine (Ser) or Threonine (Thr) residues and N-glycosylation on Aspargine (Asn) residue with the consensus sequence of Asn-X-Ser, where X is any amino acid except proline (Pro).

Glycoprotein analysis can be instrumental for understanding its structure and function and its role in the underlined diseases prognosis and their therapy. The global glycoprotein analysis market is anticipated to grow with the advancing research of glycoprotein into chronic diseases.

Coronavirus has leveraged the global glycoprotein analysis market growth. Coronavirus glycoprotein coat is actively dwelled to decode the pathogenicity and develop vaccine or therapies for the novel-virus.

Immunoblotting analysis, epitope mapping, chromatography, spectrometry and many others are actively implemented for research and glycoprotein analysis

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Glycoprotein Analysis Market: Drivers and Restraints

The global glycoprotein analysis market driver includes the massive research and technology for glycoprotein analysis to unravel their structure and function. Glycoprotein analysis has a huge market base in food consumption bacterial and yeast protein production and healthcare.

Also the advancing R&D technology and investments into glycoprotein analysis for drugs therapies vaccines disease progression understanding the biology of glycoprotein and product launches.

Glycoprotein analysis is has massive rseaech involvement into cancer of breast prostate and ovary malfunctioning glycoprotein associated enzyme and metabolism aberrations resulting in glycoprotein storage diseases including Alpha-Mannosidosis Aspartylglucosaminuria and Beta-Mannosidosis.

Glycoprotein analysis has attracted huge R&D investments subsidization and reimbursements for glycoprotein associated antibodies mutations and associated pathway targeting which altogether propels the glycoprotein analysis market growth.

However the smooth growth of glycoprotein analysis market is halted due to low abundance of native target glycoproteins purification enrichment and fractionation issues simultaneous glycoprotein sequencing glycan moieties and glycosylation sites analysis.

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Glycoprotein analysis gets complex due to the increasing heterogeneity of glycan structures. Glycoprotein analysis requires further sensitive and quantitative methods for the urgent detection of specific glycoform and its role in disease progression.

Glycoprotein analysis techniques such as spectroscopy and chemical reagents are expensive labour-intensive and the dearth of research and medical professionals decelerates the growth of glycoprotein analysis market.

Despite these constraints global glycoprotein analysis market is poised to grow lucratively due to the progressing R&D and healthcare and investments driven by the vast application of glycoprotein in healthcare and food.

The novel analysis methods with improved accuracy sensitivity and efficacy and cost-effectiveness which will propel the global glycoprotein analysis market furthermore.

Glycoprotein Analysis Market: Overview

The global glycoprotein analysis market is growing lucratively with the technological advancements of glycoprotein analysis for research healthcare and food. By glycosylation the global glycoprotein analysis glycosylation is divided into N-linkage and O-linkage which have a crucial role in the progression and therapy of several diseases.

N-glycosylation plays crucial role in arthritis cancer diabetes Crohn’s diseases and several nervous system diseases. N-linked glycoprotein is found in therapeutic antibodies including Rituximab Etanercept and Infliximab. O-glycosylation is involved in antibody structure analysis and development targeting of Alzheimer diseases and cancers by O-glycan structure and terminal Lewis epitopes.

By end-users the global glycoprotein analysis techniques have major contributions from hospital and research institutes and their collision for glycoprotein associates diseases diagnosis and analysis. The glycoprotein analysis data from research institutes are crucial for the diagnosis.

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By techniques mass spectrometry dominates the glycoprotein analysis technique due to their sensitivity specificity speed and stoichiometry for the glycoprotein identification glycosylation site and glycan structures.

Major spectrometry techniques for glycoprotein analysis include mass spectrometry (MS) Electrospray ionization mass spectrometry (ESI-MS) matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and hydrophilic interaction chromatography-mass spectrometry (HILIC-MS).

Glycoprotein Analysis Market: Regional Outlook

North America holds the largest global glycoprotein analysis market with its advanced R&D pharmaceutical development and research healthcare expenditure and investments which are supported by the government research institutes hospitals and other organizations for disease diagnosis therapy and glycoprotein food industry and key players.

Europe holds the second largest global glycoprotein analysis market with its advanced research and healthcare government’s support numerous start-ups and leading companies.

Asia-pacific glycoprotein analysis market is growing at the fastest pace fuelled by the advancing research institutes and hospitals progressing pharmaceuticals and glycoprotein analysis and production. Latin America glycoprotein analysis market is growing gradually with improving R&D and healthcare.

MEA& Africa glycoprotein analysis market is growing laggardly due to the technological backwardness although South-Africa and Israel with their advancing research and growing protein industry are expected to push the growth of regional glycoprotein analysis market

Glycoprotein Analysis Market: Key Players

The key players of the global glycoprotein analysis market include

Sigma-Aldrich. BD

Enzo Life Sciences

Creative Diagnostics

Thermo Fisher Scientific

Epitope Diagnostics Inc.

R&D Systems

QED Bioscience Inc

ACROBiosystems.

The research report presents a comprehensive assessment of the market and contains thoughtful insights facts historical data and statistically supported and industry-validated market data.

It also contains projections using a suitable set of assumptions and methodologies. The research report provides analysis and information according to market segments such as geographies application and industry.

The report covers exhaust analysis on:

Market Segments

Market Dynamics

Market Size

Supply & Demand

Current Trends/Issues/Challenges

Competition & Companies involved

Indication

Value Chain

Regional analysis includes:

North America (U.S. Canada)

Latin America (Mexico. Brazil)

Europe (Germany U.K France Italy Spain)

South Asia (India ASEAN Countries)

East Asia (Japan China & South Korea)

Oceania (Australia & New Zealand)

Middle East and Africa (GCC Countries South Africa Northern Africa)

The report is a compilation of first-hand information qualitative and quantitative assessment by industry analysts inputs from industry experts and industry participants across the value chain.

The report provides in-depth analysis of parent market trends macro-economic indicators and governing factors along with market attractiveness as per segments. The report also maps the qualitative impact of various market factors on market segments and geographies.

Report Highlights:

Detailed overview of parent market

Changing market dynamics in the industry

In-depth market segmentation

Historical current and projected market size in terms of volume and value

Recent industry trends and developments

Competitive landscape

Strategies of key players and products offered

Potential and niche segments geographical regions exhibiting promising growth

A neutral perspective on market performance

Must-have information for market players to sustain and enhance their market footprint

Glycoprotein Analysis Market: Segmentation

The global glycoprotein analysis market is segmented into glycolysation, end-users, techniques and regions.

By glycolysation

N-linkage

O-linkage

By end-users

Hospital

Research Institutes

Others

By techniques

Molecular Reagents

Mass Spectrometry

About FMI:

Future Market Insights (FMI) is a leading provider of market intelligence and consulting services, serving clients in over 150 countries. FMI is headquartered in Dubai, the global financial capital, and has delivery centers in the U.S. and India. FMI’s latest market research reports and industry analysis help businesses navigate challenges and make critical decisions with confidence and clarity amidst breakneck competition. Our customized and syndicated market research reports deliver actionable insights that drive sustainable growth. A team of expert-led analysts at FMI continuously tracks emerging trends and events in a broad range of industries to ensure that our clients prepare for the evolving needs of their consumers.

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vicky8588 · 3 years ago

Text

End Stage Renal Disease Market, Size, Share 2021-2028

Market Overview

End-stage renal diseases are associated with disorders related to the kidney and occur during chronic kidney disease, which adversely affects the functioning of the kidney.

The global end stage renal disease market size was valued US$ YY million in 2020 and is estimated to reach US$ YY million by 2028, growing at a CAGR of YY % during the forecast period (2021-2028).

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Market Dynamics

Technological advancement products developed by the market players for renal diseases are expected to drive the market growth.

Chronic kidney failure, also known as end-stage renal disease, causes the kidneys to lose function over time, which can be harmful. Most renal failure patients go to dialysis clinics numerous times a week to have their blood filtered, a time-consuming, painful, and dangerous procedure. KidneyX is a public-private partnership established by the US Department of Health and Human Services (HHS) and the American Society of Nephrology (ASN) to accelerate kidney disease prevention, diagnosis, and treatment innovation. The Kidney Project, led by Shuvo Roy, Ph.D. of UC San Francisco and William Fissell, MD of Vanderbilt University Medical Center (VUMC), successfully implanted the smartphone-sized device for the preclinical evaluation of two essential components parts of its artificial kidney, the hemofilter and the bioreactor. KidneyX's Phase 1 Artificial Kidney Prize was presented to the team, one of six winning teams chosen from a worldwide competition for this advancement.

Moreover, the Kidney Project successfully tested the hemofilter, which removes waste products and toxins from the blood and the bioreactor, which simulates other kidney functions such as blood electrolyte balancing, in independent studies. The artificial kidney developed by The Kidney Project will imitate the exceptional quality of life experienced by kidney transplant recipients—the "gold standard" of kidney disease treatment—and improve on it. Additionally, In March 2021, Baxter International Inc. announced the US Food and Drug Administration (FDA) clearance of its next-generation Artificial Kidney 98 dialysis machine, which is designed to be a portable and easy-to-use system to administer hemodialysis (HD) treatments. Therefore, it indicated that companies are taking initiatives to establish a strong footprint and launch technologically advanced products in the market.

Risks associated with the hemodialysis treatment are expected to hamper the market growth.

Certain risks associated with hemodialysis treatment increase when patients undergo HHD alone since no one is there to assist them in the event of a medical emergency. Patients may lose consciousness or become physically unable to rectify the health emergency if needles come out, blood is lost, or blood pressure is very low during solo HHD. During any health emergency, losing consciousness or becoming otherwise disabled could result in serious damage or death. Moreover, due to the length of treatment duration and the fact that therapy is administered when the patient and care partner are asleep, the dangers associated with hemodialysis treatment increase while doing nocturnal therapy. Blood access disconnects and blood loss during sleep, blood clotting due to decreased blood flow or greater treatment time, and delayed response to alarms while waking from sleep are only a few of the hazards. Therefore, ancillary devices and training are required when performing solo HHD

COVID-19 Impact Analysis

The COVID-19 outbreak has significantly impacted kidney disease incidence and management, including AKI, ESKD, GN, and kidney transplantation. Initial data from China revealed that patients with COVID-19 had a decreased risk of AKI. However, recent studies from North America show a significantly greater incidence, which is likely owing to the higher frequency of concomitant diseases such as hypertension, diabetes, and CKD. Moreover, those who receive outpatient in-center HD are in close contact with other patients and staff multiple times a week, putting them at even greater risk. Clinical presentation is atypical compared with patients not on dialysis, with patients with ESKD presenting without typical symptoms of cough and fever but fatigue and anorexia. The risk of in-hospital death is significantly higher among patients with ESKD than patients without ESKD, with older age and the need for mechanical ventilation increasing risk. Additionally, the pandemic is interrupting the medical supply chain, and many companies will vary to other geographic regions in the future to ensure that products remain available and protect their supply chain. However, the situation is expected to improve gradually in the forecast period

Segment Analysis

-By End User

· Hospital and Clinics*

· Dialysis Centres

· Others

-By Treatment And Diagnosis

Treatment

· Kidney Transplantation

Dialysis

· Peritoneal Dialysis

· emodialysis

· Wearable Artificial Kidney

Diagnosis

Blood Test

Urine Test

Imaging test

Others

Geographical Analysis

Asia-Pacific region holds the largest market share in the global end stage renal disease market

In 2020, Asia-Pacific accounted for the highest revenue share. The growth in the aging population, lifestyle stress, a large population base, improving quality of diagnosis, and growing patient affordability, presence of high disposable income and the high acceptance rate of technologically improved products and raising blood pressure and diabetes cases in the region are some of the factors due to which the market is expected to boost in the forecast period. For instance, according to the Asian Diabetics Prevention Initiatives, Asia will account for 60 percent of the world's diabetes population by 2020. A bigger contribution from China and India had 113.9 million and 65.1 million adults with diabetes, respectively. Both China and India may have nearly half a billion diabetic people by 2030.

Moreover, a report released by the Indian Government's national health portal indicates that 2.2 lakh new patients with end-stage renal disease (ESRD) are diagnosed each year, resulting in a demand for 3.4 crore dialysis treatments. In India, there were approximately 4,950 dialysis centers, most of which were in the private sector. As a result, the presence of several dialysis clinics in this region and an increase in the number of patients suffering from chronic renal and kidney illnesses are expected to boost market growth over the forecast period.

Competitive Landscape

Major key players in the end-stage renal disease market are B Braun Melsungen AG, Baxter International Inc, Medtronic PLC, Fresenius SE & Co. KGaA, Nipro Corporation, Asahi Kasei Medical Co., Trivitron Healthcare, Guangzhou MeCan Medical Limited, JMS North America Corporation and NxStage Medical. The major players are adopting several growth strategies such as product launches, acquisitions, and collaborations, which contribute to the growth of the end-stage renal disease market globally. For instance, In June 2021, Baxter launched the Sharesource Analytics 1.0 Digital Health Module to enhance the clinical management of home dialysis patients.

NxStage Medical, Inc.:

Overview:

NxStage Medical, Inc. is an American company that develops, manufactures, and markets systems to treat chronic kidney disease, acute kidney injury, and hypervolemia. It is a subsidiary of Fresenius Medical Care.

Product Portfolio:

System One: The NxStage System One is the portable hemodialysis system specifically designed for patients in their homes and is small enough to travel and bring their treatment supplies.

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#medical devices #technology

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loadguild600 · 4 years ago

Text

Biopython

Biopython Github

Biopython Tutorial

Hey, everybody loves BLAST right? I mean, geez, how can it get anyeasier to do comparisons between one of your sequences and every othersequence in the known world? But, of course, this section isn’t abouthow cool BLAST is, since we already know that. It is about the problemwith BLAST – it can be really difficult to deal with the volume of datagenerated by large runs, and to automate BLAST runs in general.

Biopython is an open-source python tool mainly used in bioinformatics field. This tutorial walks through the basics of Biopython package, overview of bioinformatics, sequence manipulation and plotting, population genetics, cluster analysis, genome analysis, connecting with BioSQL databases and finally concludes with some examples. Video created by Johns Hopkins University for the course 'Python for Genomic Data Science'. In this module, we have another long three-part lecture, this time about Communicating with the Outside, as well as a final lecture about Biopython. Biopython Development Setup We are going to use Biopython, the library for dealing with biological computation and analysis of sequences. To install it you can either use pip or conda, in case you are using Conda based distribution. Biopython uses this web address. Make no more than three requests every seconds (relaxed from at most one request every three seconds in early 2009). This is automatically enforced by Biopython. Use the optional email parameter so the NCBI can contact you if there is a problem.

Fortunately, the Biopython folks know this only too well, so they’vedeveloped lots of tools for dealing with BLAST and making things mucheasier. This section details how to use these tools and do useful thingswith them.

Dealing with BLAST can be split up into two steps, both of which can bedone from within Biopython. Firstly, running BLAST for your querysequence(s), and getting some output. Secondly, parsing the BLAST outputin Python for further analysis.

Your first introduction to running BLAST was probably via the NCBIweb-service. In fact, there are lots of ways you can run BLAST, whichcan be categorised in several ways. The most important distinction isrunning BLAST locally (on your own machine), and running BLAST remotely(on another machine, typically the NCBI servers). We’re going to startthis chapter by invoking the NCBI online BLAST service from within aPython script.

NOTE: The followingChapter BLAST and other sequence search tools describesBio.SearchIO, an experimental module in Biopython. We intend thisto ultimately replace the older Bio.Blast module, as it provides amore general framework handling other related sequence searching toolsas well. However, until that is declared stable, for production codeplease continue to use the Bio.Blast module for dealing with NCBIBLAST.

Running BLAST over the Internet¶

We use the function qblast() in the Bio.Blast.NCBIWWW module tocall the online version of BLAST. This has three non-optional arguments:

The first argument is the blast program to use for the search, as alower case string. The options and descriptions of the programs areavailable at https://blast.ncbi.nlm.nih.gov/Blast.cgi. Currentlyqblast only works with blastn, blastp, blastx, tblast andtblastx.

The second argument specifies the databases to search against. Again,the options for this are available on the NCBI Guide to BLASTftp://ftp.ncbi.nlm.nih.gov/pub/factsheets/HowTo_BLASTGuide.pdf.

The third argument is a string containing your query sequence. Thiscan either be the sequence itself, the sequence in fasta format, oran identifier like a GI number.

The qblast function also take a number of other option argumentswhich are basically analogous to the different parameters you can set onthe BLAST web page. We’ll just highlight a few of them here:

The argument url_base sets the base URL for running BLAST overthe internet. By default it connects to the NCBI, but one can usethis to connect to an instance of NCBI BLAST running in the cloud.Please refer to the documentation for the qblast function forfurther details.

The qblast function can return the BLAST results in variousformats, which you can choose with the optional format_typekeyword: 'HTML', 'Text', 'ASN.1', or 'XML'. Thedefault is 'XML', as that is the format expected by the parser,described in section Parsing BLAST output below.

The argument expect sets the expectation or e-value threshold.

For more about the optional BLAST arguments, we refer you to the NCBI’sown documentation, or that built into Biopython:

Note that the default settings on the NCBI BLAST website are not quitethe same as the defaults on QBLAST. If you get different results, you’llneed to check the parameters (e.g., the expectation value threshold andthe gap values).

For example, if you have a nucleotide sequence you want to searchagainst the nucleotide database (nt) using BLASTN, and you know the GInumber of your query sequence, you can use:

Alternatively, if we have our query sequence already in a FASTAformatted file, we just need to open the file and read in this record asa string, and use that as the query argument:

We could also have read in the FASTA file as a SeqRecord and thensupplied just the sequence itself:

Supplying just the sequence means that BLAST will assign an identifierfor your sequence automatically. You might prefer to use theSeqRecord object’s format method to make a FASTA string (which willinclude the existing identifier):

This approach makes more sense if you have your sequence(s) in anon-FASTA file format which you can extract using Bio.SeqIO (seeChapter Sequence Input/Output).

Whatever arguments you give the qblast() function, you should getback your results in a handle object (by default in XML format). Thenext step would be to parse the XML output into Python objectsrepresenting the search results (Section Parsing BLAST output),but you might want to save a local copy of the output file first. I findthis especially useful when debugging my code that extracts info fromthe BLAST results (because re-running the online search is slow andwastes the NCBI computer time).

(sec:saving-blast-output)

We need to be a bit careful since we can use result_handle.read() toread the BLAST output only once – calling result_handle.read() againreturns an empty string.

Biopython Github

After doing this, the results are in the file my_blast.xml and theoriginal handle has had all its data extracted (so we closed it).However, the parse function of the BLAST parser (describedin Parsing BLAST output) takes a file-handle-like object, sowe can just open the saved file for input:

Now that we’ve got the BLAST results back into a handle again, we areready to do something with them, so this leads us right into the parsingsection (see Section Parsing BLAST output below). You may wantto jump ahead to that now ….

Running BLAST locally¶

Introduction¶

Running BLAST locally (as opposed to over the internet, seeSection Running BLAST over the Internet) has at least major twoadvantages:

Local BLAST may be faster than BLAST over the internet;

Local BLAST allows you to make your own database to search forsequences against.

Dealing with proprietary or unpublished sequence data can be anotherreason to run BLAST locally. You may not be allowed to redistribute thesequences, so submitting them to the NCBI as a BLAST query would not bean option.

Unfortunately, there are some major drawbacks too – installing all thebits and getting it setup right takes some effort:

Local BLAST requires command line tools to be installed.

Local BLAST requires (large) BLAST databases to be setup (andpotentially kept up to date).

To further confuse matters there are several different BLAST packagesavailable, and there are also other tools which can produce imitationBLAST output files, such as BLAT.

Standalone NCBI BLAST+¶

The “new” NCBIBLAST+suite was released in 2009. This replaces the old NCBI “legacy” BLASTpackage (see below).

This section will show briefly how to use these tools from withinPython. If you have already read or tried the alignment tool examples inSection Alignment Tools this shouldall seem quite straightforward. First, we construct a command linestring (as you would type in at the command line prompt if runningstandalone BLAST by hand). Then we can execute this command from withinPython.

For example, taking a FASTA file of gene nucleotide sequences, you mightwant to run a BLASTX (translation) search against the non-redundant (NR)protein database. Assuming you (or your systems administrator) hasdownloaded and installed the NR database, you might run:

This should run BLASTX against the NR database, using an expectationcut-off value of (0.001) and produce XML output to the specifiedfile (which we can then parse). On my computer this takes about sixminutes - a good reason to save the output to a file so you can repeatany analysis as needed.

From within Biopython we can use the NCBI BLASTX wrapper from theBio.Blast.Applications module to build the command line string, andrun it:

In this example there shouldn’t be any output from BLASTX to theterminal, so stdout and stderr should be empty. You may want to checkthe output file opuntia.xml has been created.

As you may recall from earlier examples in the tutorial, theopuntia.fasta contains seven sequences, so the BLAST XML outputshould contain multiple results. Therefore useBio.Blast.NCBIXML.parse() to parse it as described below inSection Parsing BLAST output.

Other versions of BLAST¶

NCBI BLAST+ (written in C++) was first released in 2009 as a replacementfor the original NCBI “legacy” BLAST (written in C) which is no longerbeing updated. There were a lot of changes – the old version had asingle core command line tool blastall which covered multipledifferent BLAST search types (which are now separate commands inBLAST+), and all the command line options were renamed. Biopython’swrappers for the NCBI “legacy” BLAST tools have been deprecated and willbe removed in a future release. To try to avoid confusion, we do notcover calling these old tools from Biopython in this tutorial.

You may also come across Washington UniversityBLAST (WU-BLAST), and its successor,Advanced Biocomputing BLAST(AB-BLAST, released in 2009, not free/open source). These packagesinclude the command line tools wu-blastall and ab-blastall,which mimicked blastall from the NCBI “legacy” BLAST suite.Biopython does not currently provide wrappers for calling these tools,but should be able to parse any NCBI compatible output from them.

Parsing BLAST output¶

As mentioned above, BLAST can generate output in various formats, suchas XML, HTML, and plain text. Originally, Biopython had parsers forBLAST plain text and HTML output, as these were the only output formatsoffered at the time. Unfortunately, the BLAST output in these formatskept changing, each time breaking the Biopython parsers. Our HTML BLASTparser has been removed, while the deprectaed plain text BLAST parser isnow only available via Bio.SearchIO. Use it at your own risk, it mayor may not work, depending on which BLAST version you’re using.

As keeping up with changes in BLAST became a hopeless endeavor,especially with users running different BLAST versions, we now recommendto parse the output in XML format, which can be generated by recentversions of BLAST. Not only is the XML output more stable than the plaintext and HTML output, it is also much easier to parse automatically,making Biopython a whole lot more stable.

You can get BLAST output in XML format in various ways. For the parser,it doesn’t matter how the output was generated, as long as it is in theXML format.

You can use Biopython to run BLAST over the internet, as described insection Running BLAST over the Internet.

You can use Biopython to run BLAST locally, as described insection Running BLAST locally.

You can do the BLAST search yourself on the NCBI site through yourweb browser, and then save the results. You need to choose XML as theformat in which to receive the results, and save the final BLAST pageyou get (you know, the one with all of the interesting results!) to afile.

You can also run BLAST locally without using Biopython, and save theoutput in a file. Again, you need to choose XML as the format inwhich to receive the results.

The important point is that you do not have to use Biopython scripts tofetch the data in order to be able to parse it. Doing things in one ofthese ways, you then need to get a handle to the results. In Python, ahandle is just a nice general way of describing input to any info sourceso that the info can be retrieved using read() and readline()functions (seeSection What the heck is a handle?).

If you followed the code above for interacting with BLAST through ascript, then you already have result_handle, the handle to the BLASTresults. For example, using a GI number to do an online search:

If instead you ran BLAST some other way, and have the BLAST output (inXML format) in the file my_blast.xml, all you need to do is to openthe file for reading:

Now that we’ve got a handle, we are ready to parse the output. The codeto parse it is really quite small. If you expect a single BLAST result(i.e., you used a single query):

or, if you have lots of results (i.e., multiple query sequences):

Just like Bio.SeqIO and Bio.AlignIO (seeChapters Sequence Input/Outputand Multiple Sequence Alignment objects), we have a pair of inputfunctions, read and parse, where read is for when you haveexactly one object, and parse is an iterator for when you can havelots of objects – but instead of getting SeqRecord orMultipleSeqAlignment objects, we get BLAST record objects.

To be able to handle the situation where the BLAST file may be huge,containing thousands of results, NCBIXML.parse() returns aniterator. In plain English, an iterator allows you to step through theBLAST output, retrieving BLAST records one by one for each BLAST searchresult:

Or, you can use a for-loop:

Note though that you can step through the BLAST records only once.Usually, from each BLAST record you would save the information that youare interested in. If you want to save all returned BLAST records, youcan convert the iterator into a list:

Now you can access each BLAST record in the list with an index as usual.If your BLAST file is huge though, you may run into memory problemstrying to save them all in a list.

Usually, you’ll be running one BLAST search at a time. Then, all youneed to do is to pick up the first (and only) BLAST record inblast_records:

Biopython Tutorial

or more elegantly:

I guess by now you’re wondering what is in a BLAST record.

The BLAST record class¶

A BLAST Record contains everything you might ever want to extract fromthe BLAST output. Right now we’ll just show an example of how to getsome info out of the BLAST report, but if you want something inparticular that is not described here, look at the info on the recordclass in detail, and take a gander into the code or automaticallygenerated documentation – the docstrings have lots of good info aboutwhat is stored in each piece of information.

To continue with our example, let’s just print out some summary infoabout all hits in our blast report greater than a particular threshold.The following code does this:

This will print out summary reports like the following:

Basically, you can do anything you want to with the info in the BLASTreport once you have parsed it. This will, of course, depend on what youwant to use it for, but hopefully this helps you get started on doingwhat you need to do!

An important consideration for extracting information from a BLASTreport is the type of objects that the information is stored in. InBiopython, the parsers return Record objects, either Blast orPSIBlast depending on what you are parsing. These objects aredefined in Bio.Blast.Record and are quite complete.

Here are my attempts at UML class diagrams for the Blast andPSIBlast record classes. If you are good at UML and seemistakes/improvements that can be made, please let me know. The Blastclass diagram is shown in Figure fig:blastrecord.

(fig:blastrecord)

The PSIBlast record object is similar, but has support for the roundsthat are used in the iteration steps of PSIBlast. The class diagram forPSIBlast is shown in Figure Class diagram for the PSIBlast Record class..

(fig:psiblastrecord)

Fig. 1 Class diagram for the PSIBlast Record class.

Dealing with PSI-BLAST¶

You can run the standalone version of PSI-BLAST (the legacy NCBI commandline tool blastpgp, or its replacement psiblast) using thewrappers in Bio.Blast.Applications module.

At the time of writing, the NCBI do not appear to support tools runninga PSI-BLAST search via the internet.

Note that the Bio.Blast.NCBIXML parser can read the XML output fromcurrent versions of PSI-BLAST, but information like which sequences ineach iteration is new or reused isn’t present in the XML file.

Dealing with RPS-BLAST¶

You can run the standalone version of RPS-BLAST (either the legacy NCBIcommand line tool rpsblast, or its replacement with the same name)using the wrappers in Bio.Blast.Applications module.

At the time of writing, the NCBI do not appear to support tools runningan RPS-BLAST search via the internet.

You can use the Bio.Blast.NCBIXML parser to read the XML output fromcurrent versions of RPS-BLAST.

Biopython 1.76 has been released and is available from our website and PyPI.

Coming relatively soon after our last release, the timing is linked to the official end of life for Python 2, and a focus hereafter on Python 3. We intend this to be our final release supporting Python 2.7 and 3.5. Focusing on Python 3.6 or later will let us take advantage of new functionality and syntax, and simplify our code base and testing.

This release of Biopython supports Python 2.7, 3.5, 3.6, 3.7 and 3.8. It has also been tested on PyPy2.7.13 v7.1.1 and PyPy3.6.1 v7.1.1-beta0.

As in recent releases, more of our code is now explicitly available under either our original “Biopython License Agreement“, or the very similar but more commonly used “3-Clause BSD License“. See the LICENSE.rst file for more details.

In addition to the mainstream x86_64 aka AMD64 CPU architecture, we now also test every contribution on the ARM64, ppc64le, and s390x CPUs under Linux thanks to Travis CI. Further post-release testing done by Debian and other packagers and distributors of Biopython also covers these CPUs.

The Bio.motifs.PositionSpecificScoringMatrix.search() method has been re-written: it now applies .calculate() to chunks of the sequence to maintain a low memory footprint for long sequences.

Additionally, a number of small bugs and typos have been fixed with further additions to the test suite. There has been further work to follow the Python PEP8, PEP257 and best practice standard coding style, and more of the code style has been reformatted with the black tool.

Many thanks to the Biopython developers and community for making this release possible, especially the following contributors:

Chris Daley (first contribution)

Chris Rands

Christian Brueffer

Ilya Flyamer (first contribution)

Jakub Lipinski (first contribution)

Michael R. Crusoe (first contribution)

Michiel de Hoon

Peter Cock

Sergio Valqui

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